TITLE:
The Analysis of the Effective Systemic Lidocaine Dosage on the Expression of HMGB1 mRNA on Mice with Sterile Musculoskeletal Injury
AUTHORS:
Robert Hotman Sirait, Mochammad Hatta, Muhammad Ramli, Tigor Peniel Simanjuntak, Bambang Suprayogi, Andi Asadul Islam, Syafrie Kamsul Arief
KEYWORDS:
High Mobility Group Box 1, Lidocaine, Musculoskeletal Injury
JOURNAL NAME:
Open Journal of Anesthesiology,
Vol.7 No.2,
February
27,
2017
ABSTRACT: A severe injury can trigger an inflammation response
and excessive response can cause multiple organ failure. HMGB1 is an early
inflammation mediator in sterile injury and a late inflammation mediator in
infection. It is an important mediator in severe sepsis and always associated
with the severity of organ failure. Previous
studies showed that the administration of systemic lidocaine could inhibit the expression of
HMGB1 on septic mice with musculoskeletal injury. Nine male adult Balb/c mice were grouped by simple random
sampling method into three groups of intravenous
lidocaine injection dosages: 2
mg/kg, 3 mg/kg, 4 mg/kg. Musculoskeletal injury was done
by breaking the left femoral bone in a close
manner. Peripheral blood sampling was done 4 hours after injury and 2 hours
after lidocaine therap. To evaluate the expression level of HMGB1 mRNA, RT-PCR
was used. The result of our study showed that intravenous lidoaine
administration on the 3 groups could decrease the level of HMGB1.
In conclusion, lidocaine hold an important role in clinical diseases by inhibiting
HMGB1.