TITLE:
Inhibitory Activity of Burkholderia sp. Isolated from Soil in Gotsu City, Shimane, against Magnaporthe oryzae
AUTHORS:
Daniel Lemtukei, Tomoko Tamura, Quyet Thi Nguyen, Makoto Ueno
KEYWORDS:
Magnaporthe oryzae, Rice Blast, Burkholderia sp., Culture Filtrate
JOURNAL NAME:
Advances in Microbiology,
Vol.7 No.2,
February
17,
2017
ABSTRACT: An isolate GT4028 was obtained from soil samples collected from a field in Gotsu city (Kawahira), Shimane. The use of a culture suspension and culture filtrate of this isolate significantly suppressed the spore germination in Magnaporthe oryzae. The inhibitory activity of the culture filtrate was heat-stable. The formation of rice blast lesions by M. oryzae was significantly suppressed in the presence of the culture suspension of isolate GT4028. Furthermore, mycelial growth of some plant pathogenic fungi was inhibited by the isolate in a dual culture assay. Sequence analysis of 16S rDNA region of the isolate indicated that it shared similarities with species of the genus Burkholderia. Also, isolate GT4028 could be grown even in the presence of fungicides (Blastin, Kasugamycin, and Amistar) that act against M. oryzae. These results suggest that isolate GT4028 might be a potential control agent for plant protection against diseases, such as rice blast disease.