Eppell, B.A., Newell, A.M. and Brown, E.J. (1989) Adenosine Receptors Are Expressed during Differentiation of Monocytes to Macrophages in Vitro: Implications for Regulation of Phagocytosis. Journal of Immunology, 143, 4141-4145.

has been cited by the following article:

• TITLE: Anti Inflammatory Property of PDRN—An in Vitro Study on Cultured Macrophages

  AUTHORS: Chiara Castellini, Silvana Belletti, Paolo Govoni, Stefano Guizzardi

  KEYWORDS: Cell Culture, Macrophages, RAW264.7, Inflammation, Skin Aging

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.8 No.1, January 11, 2017

  ABSTRACT: Skin aging and most age-related diseases are associated with a low-grade chronic inflammation. The nucleoside adenosine, a potent endogenous anti-inflammatory agent, is deeply involved in inflammatory diseases and, by interaction with the adenosine A2 receptor (A2AR) it immediately promotes a mechanism of defence against the inflammatory damage. The aim of our study was to investigate whether polydeoxyribonucleotide (PDRN), a mixture of deoxyribonucleotides polymers of different lengths that like adenosine, binds the A2A receptor, can reduce the inflammatory state in the macrophage cell line. RAW264.7, murine macrophage cells, were incubated with PDRN in the presence and in the absence of lipopolysaccaride (LPS), which was the major component of the outer membrane of gram-negative bacteria and which acted as a strong macrophage activator. We assessed the production of nitric oxide and the secretion of inflammatory mediators (i.e., TNF-α, IL-10, IL-12 and VEGF-A). Our data showed that PDRN produced a significant decrease of inflammation in macrophages pre-stimulated with LPS, assessed in terms of the nitric oxide content (p 2A receptor, contributed to a great extent towards reducing inflammation.