TITLE:
Microcontroller-Based Sinusoidal Voltage Generation for Electrical Bio-Impedance Spectroscopy Applications
AUTHORS:
Juan A. Castro, A. Olmo, Pablo Pérez, A. Yúfera
KEYWORDS:
Sinusoidal Voltage Generator, Electrical Cell-Substrate Impedance Spectroscopy (ECIS), Bioimpedance, Microcontroller (µC), Total Harmonic Distortion (THD)
JOURNAL NAME:
Journal of Computer and Communications,
Vol.4 No.17,
December
29,
2016
ABSTRACT: A sinusoidal voltage wave generator is proposed
based on the use of micro-processor digital signals with programmable
duty-cycles, with application to real-time Electrical Cell-substrate Impedance
Spectroscopy (ECIS) assays in cell cultures. The working principle relies on the
time convolution of the programmed microcontroller (μC) digital signals. The
expected frequency is easily tuned on the bio-impedance spectroscopy range [100
Hz, 1 MHz] thanks to the μC clock frequency selection. This system has been
simulated and tested on the 8 bits μC ArduinoTM Uno with ATmega328 version.
Results obtained prove that only three digital signals are required to fit the
general specification in ECIS experiments, below 1% THD accuracy, and show the
appropriateness of the system for the real-time monitoring of this type of
biological experiments.