Morgan, K.T., Ni, H., Brown, H.R., Yoon, L., Qualls, C.W., Crosby, L.M., Reynolds, R., Gaskill, B., Anderson, S.P., Kepler, T.B., Brainard, T., Liv, N., Easton, M., Merrill, C., Creech, D., Sprenger, D., Conner, G., Johnson, P.R., Fox, T., Sartor, M., Richard,E., Kuruvilla, S., Casey, W. and Benavides, G. (2002) Application of cDNA mi croarray technology to in vitro toxicology and the selec-tion of genes for a real-time RT-PCR based screen for oxidative stress in Hep G2 cells. Toxicologic Pathology, 30, 4

has been cited by the following article:

• TITLE: Applicability of the P19CL6 cells as a model of cardiomyocytes – a transcriptome analysis

  AUTHORS: Iraj Khodadadi, Nick J. Plant, Vassilis Mersinias, Alfred E. Thumser

  KEYWORDS: Gene expression; Cardiomyocyte; P19CL6 Cell-line

  JOURNAL NAME: Health, Vol.2 No.1, January 15, 2010

  ABSTRACT: The P19CL6 cell-line, a clone of the P19 mouse embryonal carcinoma cell-line, has been exten-sively used as a model for cardiomyocytes as these cells can be differentiated into a cardio-myocyte phenotype upon incubation with di-methyl sulfoxide. Uniquely, these cells can be observed to “beat” when monitored by mi-croscopy. We started investigating the response of P19CL6 cells to fatty acids, but highly vari-able results lead us to investigate the phenotype of the P19CL6 cells in more depth. In this study we demonstrated that the P19CL6 cells are re-sponsive to adrenaline, but loose the “beating” phenotype after 16 passages in culture. Analysis of specific mRNA transcripts indicated that the P19CL6 cells express both cardiac- and skeletal muscle-specific genes, while global analysis of microarray data showed clear differences be-tween the P19CL6 cells and cardiac tissue of embryonic or adult origin. In conclusion, our observations suggest caution in the use of the P19CL6 cells as a model of cardiomyocytes unless rigorous validation for the intended analysis has been undertaken.