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has been cited by the following article:

• TITLE: Characterization of Lignin before and after Exposure to the Gastrointestinal Tract of Ruminants

  AUTHORS: E. O. S. Saliba, D. Pilo-Veloso, N. M. Rodriguez, E. A. Capanema, J. S. Saliba, A. L. C. C. Borges, L. C. Gonçalves, I. Borges, D. G. Jayme, R. R. Silva

  KEYWORDS: Lignin, Eucalyptus grandis, Ruminant

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.7 No.11, October 27, 2016

  ABSTRACT: The aim of this study was to investigate the structural composition of lignin (LIPE®) extracted from Eucalyptus grandis, before and after exposure to the gastrointestinal tract of ruminants. For this study lignin was isolated, purified and characterized using 1H and 13C Nuclear Magnetic Resonance Spectroscopy, nitrobenzene oxidation, functional groups analysis and gas chromatography with mass spectroscopy. To have a better understanding of lignin morphology and of its ultra structure, electron microscopy in wood cell wall was utilized. The lignin fecal samples showed similar 1H NMR spectrums and nitrobenzene oxidation products compared with LIPE®. The result of the microanalysis of LIPE® was similar to data shown in the literature. The ultra structure of lignin was similar to that of Bamboo (hard wood). The lignin isolated from feces of sheep was identical to the original LIPE®, which means that this product can be used as an external marker in protein digestibility in sheep.