Windham, W.R., Mertens, D.R. and Barton, F.E. (1989) Protocol for NIRS Calibration: Sample Selection and Equation Development and Validation. In: Marten, G.C., Ed., Near Infrared Reflectance Spectroscopy (NIRS): Analysis of Forage Quality, USDA Agricultural Handbook, Washington DC, 643.

has been cited by the following article:

• TITLE: Analysis of Some Important Forage Quality Attributes of Southeastern Wildrye (Elymus glabriflorus) Using Near-Infrared Reflectance Spectroscopy

  AUTHORS: Jason Brett Rushing, Uttam K. Saha, Rocky Lemus, Leticia Sonon, Brian S. Baldwin

  KEYWORDS: Acid Detergent Fiber, Digestible Dry Matter, Dry Matter Intake, Crude Protein, Near-Infrared Spectroscopy, Neutral Detergent Fiber, Wildrye

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.7 No.9, September 28, 2016

  ABSTRACT: Southeastern wildrye (Elymus glabriflorus, Vasey ex L.H. Dewey) is a cool-season, perennial grass native to southeastern United States. Recently, there is a growing interest in its development as a grazing and haying forage crop due to its wide area of adaptation across this region. Consequently, there is a great need for the evaluation of its forage quality by rapid, but accurate analytical methods like Near-Infrared Reflectance Spectroscopy (NIRS). In this study, acceptable NIRS calibration models were developed for: dry matter, DM (n = 113, R2 = 0.904, RSCD = 2.54, RSCIQ = 4.65); crude protein, CP (n = 113, R2 = 0.974, RSCD = 5.16, RSCIQ = 5.92); acid detergent fiber, ADF (n = 116, R2 = 0.896, RSCD = 2.35, RSCIQ = 1.28); neutral detergent fiber, NDF (n = 118, R2 = 0.934, RSCD = 2.53, RSCIQ = 3.38); digestible dry matter, DDM (n = 116, R2 = 0.895, RSCD = 2.36, RSCIQ = 1.35); dry matter intake, DMI (n = 115, R2 = 0.924, RSCD = 2.40, RSCIQ = 2.53); and relative feed value, RFV (n = 114, R2 = 0.932, RSCD = 2.94, RSCIQ = 2.81). Prediction of independent validation sets yielded good agreement between the NIRS predicted values and the laboratory reference values for each of: DM (n = 53, R2 = 0.831, RPD = 2.45, RPIQ = 4.24); CP (n = 57, R2 = 0.967, RPD = 5.37, RPIQ = 7.16); ADF (n = 49, R2 = 0.895, RPD = 2.97, RPIQ = 1.51); NDF (n = 53, R2 = 0.928, RPD = 3.75, RPIQ = 4.22); digestible dry matter, DDM (n = 55, R2 = 0.860, RSCD = 265, RSCIQ = 1.15); dry matter intake, DMI (n = 156, R2 = 0.845, RSCD = 2.48, RSCIQ = 2.11); and relative feed value, RFV (n = 55, R2 = 0.916, RSCD = 3.45, RSCIQ = 3.04) contents, indicating that all seven calibration models had good quantitative information. Therefore, precise, accurate, and rapid analysis of these important forage quality attributes of southeastern wildrye can be routinely done using the developed NIRS calibration models.