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has been cited by the following article:

• TITLE: Validation of an Easy Acetonitrile Fractionation for the Simplification of Protein Samples Prior to Proteomics Analyses

  AUTHORS: Yannis Karamanos, Barbara Deracinois, Gwënaël Pottiez, Sophie Duban-Deweer

  KEYWORDS: Proteomics, Acetonitrile, Fractionation

  JOURNAL NAME: Open Access Library Journal, Vol.2 No.10, October 14, 2015

  ABSTRACT: In most proteomics analyses and in particular for the “off-gel” approaches, based essentially on chromatography, the complexity of the proteome should be reduced; otherwise identifications can be hindered, especially if the mass spectrometry analysis is not conducted using state-of-the-art instrumentation. Even if the method used is a bottom-up proteomics, it appears mandatory to pre-fractionate the proteins in order to reduce the complexity. We report here the development and validation of a pre-fractionation based on the differential solubilisation of proteins using increasing concentrations of acetonitrile (ACN). This “ACN fractionation” was applied to the study of the Triton X-100 soluble sub-proteome of brain capillary endothelial cells (BCEC) with re-induced blood-brain barrier (BBB) functions.