TITLE:
Culture Filtrates of Trichoderma Isolate H921 Inhibit Magnaporthe oryzae Spore Germination and Blast Lesion Formation in Rice
AUTHORS:
Quyet Thi Nguyen, Kana Ueda, Junichi Kihara, Makoto Ueno
KEYWORDS:
Culture Filtrate, Magnaporthe oryzae, Trichoderma sp., Rice
JOURNAL NAME:
Advances in Microbiology,
Vol.6 No.7,
June
27,
2016
ABSTRACT: Spore germination and appressorium formation of Magnaporthe oryzae spores was completely suppressed by an
ethyl acetate extract of the culture filtrate from the H921 isolate
(H921-EAE-CF). Production of antifungal substance(s) in the H921-EAE-CF began
to increase up to 3 days after isolate H921 incubation. Furthermore, heat
treatment (105°C or 121°C) of H921-EAE-CF did not alter its inhibitory effect
on M. oryzae spore germination
compared to non-heat-treated H921-EAE-CF. Blast lesion formation inhibition by
H921-EAE-CF was dose-dependent. Internal transcribed spacer (ITS) region
sequence analysis indicated that this isolate shared similarities with species
of the genera Trichoderma. This study
suggests that H921-EAE-CF contains some antifungal substances that could be
promising candidates for control of rice blast disease.