TITLE:
Effects of Nourishing “Yin”-Removing “Fire” Chinese Herb Mixture on the Expression of GABAB Receptors in Hypothalamus of Precocious Puberty Female Rats
AUTHORS:
Zhejun Chen, Zehui Meng, Shiran Wang, Liting Zhu, Zhanzhuang Tian
KEYWORDS:
Precocious Puberty, GABAB Receptors, Chinese Herb Medicine, Danazol, Rats
JOURNAL NAME:
Chinese Medicine,
Vol.7 No.2,
June
15,
2016
ABSTRACT: Objectives: To investigate the potential role of GABAB Receptors (GABABRs)
involved in the effect of Nourishing “Yin”-Removing “Fire” Chinese herb mixture
(TCM) treatment on precocious puberty. Methods: Female Sprague-Dawley rats were
randomly divided into four groups: normal (N), central precocious puberty (CPP)
model (M), CPP fed with normal saline (S) and CPP fed with Nourishing
“Yin”-Removing “Fire” Chinese herb mixture (TCM). Rats of postnatal day 5 were
given a single subcutaneous injection of 240 μg danazol to establish CPP model
rats. Rats of S and TCM groups were continuously administered with saline or
nourishing “Yin”-removing “Fire” Chinese herb mixture since postnatal day 15.
The expression of GABABRs was detected by means of real-time PCR and
immunohistochemistry. Results: The expression of hypothalamic GnRH mRNA in M was
significantly increased on the day of pre-puberty when compared with that of N (P
B1 receptor (GABABR1) immunoreactive cells in the arcuate nucleus (ARN) was
decreased in M when compared with that of N (P B2 receptor
(GABABR2) immunoreactive cells in the ARN, MS and diagnonal band of broca (DBB)
were decreased in M when compared with those of N (P