TITLE:
Expression Analysis of Aquaporin-1 (Aqp-1) in Human Biliary Tract Carcinoma
AUTHORS:
Shinichi Sekine, Tomoyuki Okumura, Takuya Nagata, Kazuto Shibuya, Isaku Yoshioka, Koshi Matsui, Ryouta Hori, Kazuhiro Tsukada
KEYWORDS:
Aquaporin, Biliary Tract Carcinoma, Tissue Microarray, Immunohistochemistry
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.7 No.1,
January
12,
2016
ABSTRACT: Background: Aquaporins (AQPs) are important in controlling bile water
secretion. AQP is related to the invasion and metastasis of cancer. However,
the relationship of biliary tract cancer is not clear. The role of AQP-1 in
cancer cell is also unknown. Methhods: We analyzed AQP-1 expression using
tissue microarray (TMA) in 99 samples immunohistochemically (50 gallbladder
carcinoma, 39 bile duct carcinoma and 10 Papilla Vater carcinoma patients who
underwent surgery at our department from 1997 to 2011). Gene expressions were
evaluated by the combination of the immunohistological intensity and
distribution. The expression level is compared to the clinico-pathological data
of the patients. Results: In the TMA, depth of tumor invasion and histological
type are associated with AQP-1 expression. The group of patients with high AQP-1
expression is associated with higher rates of disease specific survival
(log-rank p = 0.013). Cox’s proportional hazard model reveals that AQP-1
expression is an independent prognostic factor (RR, 0.324; p = 0.001) in
multivariate analysis. There is a correlation between AQP-1 expression and
tumor invasion. Conclusions: These observations of this study suggest that AQP-1
expression may be favorable biomarkers associated with prognosis and tumor
invasion in biliary tract carcinoma.