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http://dx.doi.org/10.1016/S0190-9622(96)90349-7

has been cited by the following article:

• TITLE: Stability Indicating RP-HPLC Method for Estimation of Impurities of Vitamin D3 Analogue and Corticosteroid Used as Antipsoriatic Drugs. An Attempt to Characterize Pre-Calcipotriene

  AUTHORS: R. K. Bhogadi, A. Satyanarayana, N. Someswara Rao, Srinivas Arutla, A. Malleswara Reddy

  KEYWORDS: Calcipotriene, Pre-Calcipotriene, HPLC, Photo Isomers, Ointment

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.6 No.13, December 25, 2015

  ABSTRACT: A single RP-HPLC method is developed for estimation of isomeric impurities of vitamin D3 analogue-Calcipotriol/Calcipotriene (Calci) and impurities of Betamethasone dipropionate (BD). The developed method is capable of separating impurities of Calci and BD, specifically pre-Calcipotriene (Pre-Calci) from other known and unknown impurities. Pre-Calci is isolated and is characterised using few analytical techniques. These impurities are separated using a RP-C18 150 × 4.6 mm, 2.7 μm column maintained at 50°C. The mobile phase consisted of mixture of water, methanol, acetonitrile and tetrahydrofuran eluted in gradient mode. Detection was done at 264 nm and 240 nm for Calci and BD impurities respectively. The method can be used for determining quality of Calci and BD drugs and ointment based drug products. It is stability indicating related substance method for both the drugs and drug products.