Issa, Y., Watts, D.C., Brunton, P.A., Waters, C.M. and Duxbury, A.J. (2004) Resin composite monomers alter MTT and LDH activity of human gingival fibroblasts in vitro. Dental Materials, 20(1), 12-20.

has been cited by the following article:

• TITLE: In-Vitro Cellular Responses of Human Dental Primary Cells to Dental Filling Restoratives

  AUTHORS: Jun Sun, Yiming Weng, Fengyu Song, Dong Xie

  KEYWORDS: In vitro cytotoxicity; human pulp cells; human gingival fibroblasts; 3T3 mouse fibroblast cells; dental cement; resin composite.

  JOURNAL NAME: Journal of Biomaterials and Nanobiotechnology, Vol.2 No.3, May 20, 2011

  ABSTRACT: In vitro cytotoxicity of six contemporary commercial dental filling restoratives on human dental primary cells, pulp cells (HPCs) and human gingival fibroblasts (HGFs), were tested using WST-1 assay. Continuous 3T3 mouse fibroblast cell lines were used for comparison. The results show that conventional glass-ionomer cement (GIC) Fuji II is not cytotoxic to all the cells. Resin-modified GIC (RMGIC) Fuji II LC is not cytotoxic to both HPCs and HGFs but cytotoxic to 3T3 cells. RMGIC Vitremer and resin composite Z100 are very cytotoxic to all the cells. Resin composite P60 is cytotoxic but much less cytotoxic than Z100. Polycarboxylate cement Durelon is the most cytotoxic among the six tested materials. It was found that continuous 3T3 cell lines were more vulnerable to leachable cytotoxic components than primary HPCs and HGFs. It was also found that the cytotoxcity of the tested materials was dose-dependent.