TITLE:
Clonal Fidelity of Micropropagated Psidium guajava L. Plants Using Microsatellite Markers
AUTHORS:
Bernessa Rawls, Karen Harris-Shultz, Sadanand Dhekney, Ivis Forrester, Viji Sitther
KEYWORDS:
Genetic Stability, Guava, Simple Sequence Repeats, Tissue Culture
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.6 No.14,
September
23,
2015
ABSTRACT: Micropropagation of Psidium guajava L. (guava) is a viable alternative to currently adopted techniques
for large-scale plant propagation of commercial cultivars. Assessment of clonal fidelity in
micropropagated plants is the first step towards ensuring genetic uniformity in mass production
of planting material. In the present study, 31 plants of guava cultivar “Lucknow 49” regenerated by
micropropagation were tested for genetic fidelity by comparing them to the mother plant from
which explant material was obtained. Efficient rooting of in vitro proliferated shoots was obtained
by culture on 1/2 strength MS medium supplemented with either 9.8 μM indole butyric acid (IBA)
or 11.4 μM indole acetic acid (IAA). Leaf samples of 31 regenerated plants were compared to the
mother plant using 17 simple sequence repeat (SSR) markers. While 16 SSRs detected the same
allele, locus mPgCIR07 detected slight differences, where six micropropagated plants were 1 bp
smaller (152 bp) than the parental genotype (153 bp). Differences in leaf tissues for anthocyanin
pigmentation were also noted among micropropagated plants. Results of the study indicated efficient
rooting of “Lucknow-49” cultivar for rapid propagation of planting material, and revealed
that micropropagated plants were identical for 16 of the 17 loci examined. Although most mutations
induced by tissue culture may not have an effect on phenotype, the possibility that novel
phenotypes can be generated in a commercial setting exists.