TITLE:
Antiamylase Potential of Telfairia occidentalis Leaves from Cameroon and Effect of Their Dietary Supplementation on Fasting Blood Glucose in Wistar Rats
AUTHORS:
Palla Nyamen Corine Leslie, Etoundi Omgba Cunégonde Blanche, Gouado Gouado Inocent
KEYWORDS:
Antiamylase Potential of Telfairia occidentalis Leaves from Cameroon and Effect of Their Dietary Supplementation on Fasting Blood Glucose in Wistar Rats
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.3 No.9,
September
17,
2015
ABSTRACT:
The study of edible plants,
especially in developing countries, might provide more affordable means for the
management of diabetes. Telfairia occidentalis is one of the plants whose
leaves are commonly consumed in Cameroon. This work hereby studied the
antiamylasic potential of its aqueous leaves extract and the effect of its
dietary supplementation on fasting blood glucose in Wistar rats. An aqueous
extract (1:6) was prepared from shed-dried T. occidentalis leaves by maceration.
Its antiamylase activity was evaluated in vitro and a phytochemical screening
was realized. Its acute toxicity and its effect on an oral glucose tolerance
test (OGTT) were evaluated in rats. The effect of T. occidentalis leaves
dietary supplementation (10%) on fasting blood glucose was studied for 28 days
in rats fed with carbohydrate enriched diet, using Glibenclamide (0.3 mg/kg
body weight) as reference hypoglycemic drug. Results showed that there was
total inhibition of α-amylase activity in vitro by T. occidentalis aqueous
leaves extract at 0.075 mg/ml. The presence of tannins, flavonoids and
anthocyanins was revealed by the phytochemical screening. No sign of toxicity
was observed in rats after an oral administration of the extract at 2000 mg/kg
body weight. The extract significantly hindered a rise in blood glucose at 400
mg/kg body weight during an oral glucose tolerance test. Dietary
supplementation with T. occidentalis leaves caused a significant decrease (p