TITLE:
Inhibition of Magnaporthe oryzae by Culture Filtrates of Fungi Isolated from Wild Mushrooms
AUTHORS:
Quyet Nguyen Thi, Kana Ueda, Junichi Kihara, Makoto Ueno
KEYWORDS:
Rice Blast Disease, Culture Filtrate, Antifungal Compound, Magnaporthe oryzae, Mushroom
JOURNAL NAME:
Advances in Microbiology,
Vol.5 No.10,
September
9,
2015
ABSTRACT: Fungal strains isolated from the fruiting bodies of wild mushrooms were
evaluated for fungicidal activity against Magnaporthe
oryzae, the causal agent of the rice blast disease. Fungal isolates (n =
105) were obtained from 46 samples of wild mushrooms. Infection behaviors of M. oryzae were assessed in the presence
of culture filtrates from 90 fungal isolates, of which 20 inhibited spore
germination. Heat-treated culture filtrates of these isolates were classified
into 3 groups according to biological activity. Blast lesion formation by M. oryzae was significantly inhibited by
pretreatment with culture filtrates from 4 fungal isolates. ITS region sequence
analysis indicated that theseisolates shared similarities with species of the genera Annulohypoxylon, Nigrospora, and Penicillium. Studies
of symbiotic and parasitic fungi from wild mushrooms may yield potential
control agents for plant diseases such as the rice blast disease.