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http://dx.doi.org/10.1016/0031-9422(75)85135-1

has been cited by the following article:

• TITLE: Cannabinoids Production by Hairy Root Cultures of Cannabis sativa L.

  AUTHORS: Sayed Farag, Oliver Kayser

  KEYWORDS: Tetrahydrocannabinol, Cannabinoids, Callus, Hairy Root, Cannabis sativa

  JOURNAL NAME: American Journal of Plant Sciences, Vol.6 No.11, July 31, 2015

  ABSTRACT: Tetrahydrocannabinol (THC) derivatives are used clinically as analgesic, anti-inflammatory, appetite stimulant, anti-emetic and anti-tumor cannabinoids. THC and its related compounds are at present obtained by extraction from intact Cannabis plants or chemical synthesis, but plant cell cultures may be an alternative source of production. In the present study, hairy root cultures of C. sativa (Cannabaceae) were induced by incubation of aseptically grown callus culture with solid B5 medium supplemented with 4 mg/l naphthaleneacetic acid in darkness at 25°C. Hairy root growth profiles in shake flask, increased periodically during 35 days of growth cycle. The cannabinoid contents produced in minor levels and remained below 2.0 μg/g dry weight. The contents of can-nabinoid were analyzed by liquid chromatography and confirmed by mass spectrometry.