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http://dx.doi.org/10.1128/AEM.00650-10

has been cited by the following article:

• TITLE: Environmental Toxicity and Antimicrobial Efficiency of Titanium Dioxide Nanoparticles in Suspension

  AUTHORS: Muriel Bonnet, Christophe Massard, Philippe Veisseire, Olivier Camares, Komla Oscar Awitor

  KEYWORDS: Photokilling Activity, Titanium Nanoparticles, E. coli, L. c. rhamnosus, S. aureus, Hydrogen Peroxide

  JOURNAL NAME: Journal of Biomaterials and Nanobiotechnology, Vol.6 No.3, July 17, 2015

  ABSTRACT: The aim of this work was to evaluate the photokilling efficiency of synthesized titanium dioxide nanoparticles in suspension. Two strains of Escherichia coli, Lactobacillus casei rhamnosus and Staphylococcus aureus were used as probes to test the photokilling activities of the nanoparticles. The toxicity effects of TiO2 nanoparticles on the environment were determined by a standard test using gram-negative bioluminescent bacteria Vibrio fischeri. The antimicrobial activity of these nanoparticles (NPs) was then investigated versus NPs concentration, UV irradiation time and micro- organism strains. We evaluated the LC50 values of the nanoparticles suspension by counting the Colony-Forming Units. Results highlighted the differences in bacteria sensitivity facing photokilling treatment induced by the irradiation of anatase TiO2 nanoparticles suspension. At the concentration of 1 g·L-1 TiO2, tested bacteria were killed after 30 minutes of photo-treatment. Using different TiO2 concentrations, the Staphylococcus aureus gram-positive/catalase-positive bacteria were more resistant than gram-negative/catalase-positive ones or gram-positive/catalase-negative bacteria. An effect of UV irradiation was evaluated by the quantification of hydrogen peroxide generated by the photolysis of water molecules in presence of the nanoparticles with or without the most resistant bacterium (S. aureus). After 30 minutes with UV irradiation in these two conditions, the concentration of hydrogen peroxide was 35 μM in presence of 1.2 g·L-1 TiO2 suspension. This result suggested that the resistance mechanism of S. aureus was not due to an extracelullar H2O2 enzymatic degradation.