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http://dx.doi.org/10.1016/j.mycres.2005.10.006

has been cited by the following article:

• TITLE: Optimization of Mycelial Biomass Production in Submerged Culture Fermentation of Pleurotus flabellatus Using Response Surface Methodology

  AUTHORS: Shaiful Azuar Mohamad, Mat Rasol Awang, Rosnani Abdul Rashid, Liew Siew Ling, Fauzi Daud, Aidil Abdul Hamid, Rahayu Ahmad, Wan Mohtar Wan Yusoff

  KEYWORDS: Submerged Culture Fermentation, Response Surface Methodology, Mushroom Production

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.6 No.6, June 26, 2015

  ABSTRACT: The factors selected to optimize the productivity of Pleurotus flabellatus biomass in 250ml working volume Erlenmeyer flask were agitation rate, initial pH value and incubation temperature. The central composite design was applied to study the significant factors and the interactions between the chosen factors, if present. The Design Expert software generated 20 runs. The optimized conditions obtained were as follows: the agitation rate of 129.8 rpm, incubation temperature at 27.8°C, and initial pH of 6.06. The optimized conditions tripled the productivity at the range of 980 - 1040 mg/litre/day compared to the initial rate productivity at 310 mg/litre/day. From the quadratic equation,the agitation rate, temperature and the interaction between agitation rate and temperature were found to be significant (p