Rosati, S., Ortoffi, M., Profiti, M., Mannelli, A., Mignone, W., Bollo, E. and Gradoni, L. (2003) Prokaryotic Expression and Antigenic Characterization of Three Recombinant Leishmania Antigens for Serological Diagnosis of Canine Leishmaniasis. Clinical and Vaccine Immunology, 10, 1153-1156. - References

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Rosati, S., Ortoffi, M., Profiti, M., Mannelli, A., Mignone, W., Bollo, E. and Gradoni, L. (2003) Prokaryotic Expression and Antigenic Characterization of Three Recombinant Leishmania Antigens for Serological Diagnosis of Canine Leishmaniasis. Clinical and Vaccine Immunology, 10, 1153-1156.

has been cited by the following article:

TITLE: The Serological Diagnosis of Canine Leishmaniasis: Commercial ELISA or in House IFAT?

AUTHORS: Pasquale Santoro, Andrea Vellusi

KEYWORDS: ELISA, IFAT, Leishmania infantum

JOURNAL NAME: Open Journal of Veterinary Medicine, Vol.5 No.2, February 13, 2015

ABSTRACT: The levels of anti-Leishmania infantum antibodies are relevant with the diagnosis and the followup of infected dogs. Both ELISA (Enzyme-Linked Immunosorbent Assay) and IFAT (Indirect Immuno-Fluorescent Antibody Test) may be employed to estimate the antibody levels in dogs, but at present, the correlation between ELISA absorbance and IFAT titer has not been investigated. In this paper, we compared the optical densities obtained with a commercial ELISA, Leiscan?(Laboratorios Dr. Esteve S.A.), versus the titers obtained by in house IFAT. We measured ELISA absorbance of: 44 IFAT negative samples coming from an endemic area; 10 negative samples coming from a non endemic area; 29 samples with an IFAT titer between 1/40 and 1/80; 10 samples with an IFAT titer of 1/160; 9 samples with an IFAT titer of 1/320; 10 samples with an IFAT titer of 1/640; 10 samples with an IFAT titer ≥ of 1/1280. Results show that: a) there is a poor correlation between IFAT titer and ELISA absorbance; b) Leiscan? is not able to distinguish between IFAT negative samples and IFAT titers up to 1:160; c) IFAT negative samples from an endemic or non endemic area show different ELISA absorbance; d) the performance of the kit may be improved by the use of a more appropriate cut-off.