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http://dx.doi.org/10.1073/pnas.082089499

has been cited by the following article:

• TITLE: Human Nail Clippings as a Source of DNA for Genetic Studies

  AUTHORS: Le Truong, Hannah Lui Park, Seong Sil Chang, Argyrios Ziogas, Susan L. Neuhausen, Sophia S. Wang, Leslie Bernstein, Hoda Anton-Culver

  KEYWORDS: Single Nucleotide Polymorphism (SNP), Nail Clippings, Genotyping, Whole Genome Amplification (WGA)

  JOURNAL NAME: Open Journal of Epidemiology, Vol.5 No.1, January 21, 2015

  ABSTRACT: Blood samples have traditionally been used as the main source of DNA for genetic analysis. How-ever, this source can be difficult in terms of collection, transportation, and long-term storage. In this study, we investigated whether human nail clippings could be used as a source of DNA for SNP genotyping, null-allele detection, and whole-genome amplification. From extracted nail DNA, we achieved amplicons up to a length of ~400 bp and >96% concordance for SNP genotyping and 100% concordance for null-allele detection compared to DNA derived from matched blood sam-ples. For whole-genome amplification, OmniPlex performed better than Multiple Displacement Amplification with a success rate of 89.3% and 76.8% for SNP genotyping and null-allele detection, respectively. Concordance was ~98% for both methods. When combined with OmniPlex whole-genome amplification, human nail clippings could potentially be used as an alternative to whole blood as a less invasive and more convenient source of DNA for genotyping studies.