TITLE:
Application of Total Error Strategy in Validation of Affordable and Accessible UV-Visible Spectrophotometric Methods for Quality Control of Poor Medicines
AUTHORS:
Jérémie Kindenge Mbinze, Jean Nsangu Mpasi, Elza Maghe, Sébastien Kobo, Rodrigue Mwanda, Guy Mulumba, Jacquie Bolavie Bolande, Trésor Menanzambi Bayebila, Maguy Borive Amani, Philippe Hubert, Roland Djang’eing’a Marini
KEYWORDS:
Poor Quality Medicines, UV-Visible Spectrophotometric Methods, Quinine, Amoxicillin, Metronidazole, Validation, Total Error, Accuracy Profiles
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.6 No.2,
January
19,
2015
ABSTRACT: In the framework of fighting against the poor quality medicines sold in developing countries using classical analytical methods easily accessible in those countries, four UV-Visible spectrophotometric methods for one antimalarial (quinine) and two antibiotics (amoxicillin and metronidazole) have been developed and validated according to the total error strategy using the accuracy profiles as a decision tool. The dosing range was 2 - 10 μg/mL (for quinine sulfate in tablet), 4 - 12 μg/mL (for quinine bichlorhydrate in oral drop-metronidazole benzaote in oral suspension) and 15 - 35 μg/mL (for amoxicillin trihydrate in capsule). The validated methods were then applied in determining the content of some analogous medicines sold in the Democratic Republic of Congo. Thus, the proposed UV-Visible spectrophotometric methods are simple and suitable to quantify quinine, amoxicillin and metronidazole in different pharmaceutical forms.