Pukalskas, A., Rimantas Venskutonis, P., Salido, S., De Waard, P. and Van Beek., T.A. (2012) Isolation, Identification and Activity of Natural Antioxidants from Horehound (Marrubium vulgare L.) Cultivated in Lithuania. Food Chemistry, 130, 695-701.
http://dx.doi.org/10.1016/j.foodchem.2011.07.112

has been cited by the following article:

• TITLE: Optimization of Extraction Conditions of Some Phenolic Compounds from White Horehound (Marrubium vulgare L.) Leaves

  AUTHORS: Karim Bouterfas, Zoheir Mehdadi, Djamel Benmansour, Meghit Boumedien Khaled, Mohamed Bouterfas, Ali Latreche

  KEYWORDS: Marrubium vulgare, Phenolic Compounds, Optimization, Extraction Conditions

  JOURNAL NAME: International Journal of Organic Chemistry, Vol.4 No.5, December 26, 2014

  ABSTRACT: This research was aimed to optimize the extraction conditions of three phenolic compounds: total phenolics, flavonoids and condensed tannins, from White Horehound’s leaves (Marrubium vulgare L.). Distilled water and different organic solvents such as: methanol, ethanol and acetone, were used, with various concentrations (20% - 80%, v/v), temperatures (20°C - 60°C) and extraction times (30 - 450 min). Results showed that the maximum total phenolics amounts (293.34 ± 14.60 mg gallic acid equivalent/g dry weigh), were obtained with 60% aqueous methanol at 25°C for 180 min; total flavonoids (79.52 ± 0.55 mg catechin equivalent/g dry weigh) with 80% aqueous methanol at 20°C for 450 min, and condensed tannins (28.15 ± 0.80 mg catechin equivalent/g dry weigh) with 60% aqueous acetone at 50°C and for 180 min. ANOVA test showed the significant effect (***P