Matsui, M., Motomura, D., Fujikawa, T., Jiang, J., Takahashi, S., Manabe, T. and Taketo, M.M. (2002) Mice Lacking M₂ and M₃ Muscarinic Acetylcholine Receptors Are Devoid of Cholinergic Smooth Muscle Contractions But Still Viable. Journal of Neuroscience, 22, 10627-10632.

has been cited by the following article:

• TITLE: Expression Imbalance of Cholinergic M2 and M3 Receptors Contributes to the Motility Reduction of the Small Intestine in Spleen Qi Deficiency

  AUTHORS: Fan Zhao, Xudong Liu, Wenjun Liu, Jiyan Cai, Lingzhi Wang, Huaxin Yu, Lu Wang, Lide Zhang, Deshan Wang, Dehong Shan

  KEYWORDS: Spleen Qi Deficiency, Small Intestine Motility, M2 Receptor, M3 Receptor

  JOURNAL NAME: Chinese Medicine, Vol.5 No.4, December 10, 2014

  ABSTRACT: Objective: To study roles of cholinergic M2 and M3 receptors in the motility reduction of small intestine (SI) in spleen qi deficiency. Methods: 16 male SD rats were randomly divided in the control group and spleen qi deficiency group (model group)—8 rats each group; spleen qi deficiency model of the improper diet and overfatigue was established; the SI propelling rate (SIPR) was used to evaluate the SI motility; ELISA was used to measure concentrations of acetylcholine (ACh), cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) in the SI tissue; immohistochemistry was employed to detect expressions of cholinergic M2 and M3 receptors. Results: Compared with those in the control group, SIPR was reduced; expression of M2 receptors was increased; and expression of M3 receptors and concentrations of cAMP and PKA were decreased, significantly, in the model group. Conclusions: Expression imbalance of cholinergic M2 and M3 receptors might contribute to the motility reduction of the SI in spleen qi deficiency.