Xu, L.S., Zhang, Z.B., Song, M.J., Chao, X.P., Wang, F.S. and Zhang, C.G. (2002) A Method of Extracting Genomic DNA from Animal Specimen Preserved in Formalin. Acta Zoologica Sinica, 48, 264-269.

has been cited by the following article:

• TITLE: DNA Extraction from Formalin Fixed Coilia macrognathos Fin Tissues

  AUTHORS: Xiping Yuan, Hong Li, Chongrui Wang, Bo Hong

  KEYWORDS: Formalin, Fish Fin, Mitochondria DNA

  JOURNAL NAME: Agricultural Sciences, Vol.5 No.12, October 22, 2014

  ABSTRACT: Approximately 200 mg of fin tissue from each specimen was cut into pieces and treated with a gradient of ethanol (65%, 70%, 75%, 80%, 85%, 90%, 95% and 100%) and then DNA was extracted from formalin fish fin tissues. Agarose gel visualization of the DNA confirmed DNA extracted. MtDNA cyt b gene banding pattern of samples with agarose gel was clearly visible and could be used to be sequenced. In terms of DNA purity, the 260/280 ratio of 87.5% samples ranged between 1.8 and 2.0, indicating that DNA of the majority of the samples was pure. The developed DNA extraction procedure from formalin fish fin tissues by pretreatment with a gradient of ethanol system will be a useful tool to study the genetic structure and phylogenesis of endangered fish, by specimens preserved formalin-fixed in museum and herbarium.