TITLE:
Proteomic Analysis of Celecoxib on Chondrocytes from Patients with Osteoarthritis
AUTHORS:
Kenji Takenouchi, Mitusmi Arito, Toshiyuki Sato, Kenji Takahashi, Manae S. Kurokawa, Kazuo Yudoh, Shinro Takai, Tomohiro Kato, Hiroshi Nakamura
KEYWORDS:
Proteomic Analysis, Celecoxib, Chondrocytes, Osteoarthritis
JOURNAL NAME:
Modern Research in Inflammation,
Vol.3 No.3,
July
29,
2014
ABSTRACT:
Objective: To study
a comprehensive proteomic analysis of celecoxib in oseteoarthritis (OA) chondrocytes.
Methods: OA chondrocytes were stimulated with celecoxib, IL-1β and IL-1β together with
celecoxib. Proteins were extracted from the cells and subjected to
2-dimensional differential image gel electrophoresis (2D-DIGE). Proteins of
interest were identified by mass spectrometry. Results: Eighty-six protein
spots showed significantly different intensities with each reagent or reagent
combination. AAA+ protein, HSP47/Serpin, cAMP-dependent protein kinase type
II-beta regulatory subunit, alpha-actin-4 and tubulin decreased with the
addition of celecoxib, while apolipoprotein A-V, glutamate carboxipeptide 2,
mitochondrial stress-70 protein, sorting nexin-9 and GRP78 increased with the
addition of celecoxib. GRP78 is a stress protein and may be chondroprotective.
Celecoxib modulated IL-1β stimulated
chondrocytes, and CD200R and moesin were identified as such resulting proteins.
Conclusion: Protein profiles of OA chondrocytes changed after administration of
celecoxib. Further investigation is needed to elucidate the function of each
protein in OA chondrocytes.