TITLE:
Phylogenetic Position of North Sulawesi Tarsius sp. Based on Partial Cytochrome b Gene Sequences
AUTHORS:
Decky David Wemvrid Kamagi, Aloysius Duran Corebima, Mariana Rengkuan
KEYWORDS:
Molecular Phylogeny, Tarsius sp., Sulawesi, Cyt b Gene, Partial Sequence
JOURNAL NAME:
Open Journal of Genetics,
Vol.4 No.4,
July
23,
2014
ABSTRACT:
Cyt
b gene of North Sulawesi Tarsius sp., T. tumpara, T. sangirensis and T. tarsier (T. spectrum) had been
partially sequenced. The homologous sequence of three groups had been compared
to describe the phylogenetic position among them, as well as several other
species accessed from the Genbank. Total DNA extracted from the muscular tissue
had been obtained through tail cut sampling using the innuPREP DNA micro kit,
and amplified using a pair of universal primer, L14841 and H15149. The size of
the cyt b gene sequence amplified was 307 bp long. Sequence aligned using
CLUSTAL-X program and diversity analysis were done using version 5.2.2. MEGA5
program. Genetic distance had been calculated by Tamura 3 parameter method and
phylogenetic tree had been built using Neighbor-Joining and Maximum Likelihood
methods. Genetic distance based on cyt b gene nucleotide was found from 0 to 0.240
with an average of 0.080. The phylogenetic tree constructed by Neighbor
Joining and Maximum Likelihood methods indicated that T. tarsier, T. sangirensis and T. tumpara were closely related with Tarsius tarsier-complex, and distantly
related with Cephalopachus bancanus and Carlito syrichta. The genetic
distance and the phylogenetic tree had been constructed on the base of partial
cyt b gene sequence of T. tarsier, T. sangirensis, T. tumpara and 5 other tarsier species and their accession. Those results are
consistent with taxonomy based on morphology and vocal acoustic form.