TITLE:
The Development and Application of an Indirect ELISA Test for the Detection of Chicken Anaemia Virus (CAV) by VP1 in Chicken Flock Serum
AUTHORS:
Elham O. Mahgoub
KEYWORDS:
Indirect ELISA, Commercial ELISA, Chicken Anaemia Virus, Recombinant Protein VP1, Sensitivity, Specificity, Standard Curve for Indirect ELISA
JOURNAL NAME:
Open Journal of Genetics,
Vol.4 No.4,
July
23,
2014
ABSTRACT:
Chicken anaemia
virus (CAV) causes a viral disease in chickens worldwide and thus has economic
importance. The main aim of this study was to develop a rapid, sensitive and
specific VP1-CAVI indirect ELISA for the detection of CAV infection. The CAV-VP1,
was separately cloned and expressed in recombinant E. coli. The purified
recombinant CAV-VP1 protein was then coated as an antigen on an ELISA plates to
evaluate its reactivity against chicken sera. The resulting indirect ELISA was
then compared with a commercial ELISA. The specificity and sensitivity of the
indirect ELISA were measured as 93.3% and 100%, respectively. A t-test produced a t-value of 15.805 for the indirect
ELISA and revealed a significant difference between CAV-positive
serum and CAV-negative serum (p-value of 0.001). For the second variable (i.e., a commercial ELISA), the t-test
yielded a t-value of 5.063, which revealed a significant difference between
CAV-positive serum and CAV-negative serum (p-value of 0.015). This intervention
produces statistically significant improvements in both variables (p-values r = 0.93. Therefore, this work
can be considered as a new achievement in diagnosis for Chicken anaemia virus
in chicken flocks.