Thokoane, L.N. and Rutherford, R.S. (2001) cDNA-AFLP Differential Display of Sugarcane (Saccharum spp., Hybrids) Genes Induced by Challenge with the Fungal Pathogen Ustilago scitaminea (Sugarcane Smut). Proceedings of the South African Sugar Technologists Association, 75, 104-107.

has been cited by the following article:

• TITLE: Sugarcane Transcript Profiling Assessed by cDNA-AFLP Analysis during the Interaction with Sugarcane Mosaic Virus

  AUTHORS: Cibele N. F. Medeiros, Marcos C. Gonçalves, Ricardo Harakava, Silvana Creste, Paula M. Nóbile, Luciana R. Pinto, Dilermando Perecin, Marcos G. A. Landell

  KEYWORDS: Plant Resistance, Plant Viruses, Molecular Markers, Candidate Genes, cDNA-AFLP, SCMV

  JOURNAL NAME: Advances in Microbiology, Vol.4 No.9, July 11, 2014

  ABSTRACT: Sugarcane mosaic caused by Sugarcane Mosaic Virus (SCMV) is one of the most important virus diseases of sugarcane. In the present study, changes in the transcription profile obtained by cDNA-AFLP analysis were investigated in two sugarcane varieties contrasting to SCMV resistance, when challenged with a severe virus strain. Healthy plants derived from meristem tip tissue culture were mechanically inoculated under greenhouse controlled conditions and sampled at 24, 48 and 72 hours after inoculation. A total of 392 transcript-derived fragments (TDFs) were verified in the resistant variety against 380 in the susceptible one. The two sugarcane genotypes showed differential behavior in the number of induced and repressed TDFs along the time-course samplings. Ten out of 23 sequenced TDFs (unique from the resistance variety), showed identity with known plant sequences, mostly related to plant defense mechanisms against pathogens. The cDNA-AFLP technique was effective in revealing changes in the transcription profile within and between contrasting varieties when challenged by SCMV.