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Steinman, R.M., Machtinger, B.G., Fried, J. and Cohn, Z.A. (1978) Mouse Spleen Lymphoblasts Generated in Vitro. Recovery in High Yield and Purity after Floatation in Dense Bovine Plasma Albumin Solutions. The Journal of Experimental Medicine, 147, 279-296.
http://dx.doi.org/10.1084/jem.147.2.279

has been cited by the following article:

  • TITLE: Cell Development in Primary Culture of Porcine Bone Marrow

    AUTHORS: Zaven Karalyan, Lusine Simonyan, Alla Misakyan, Liana Abroyan, Lina Hakobyan, Aida Avetisyan, David Saroyan

    KEYWORDS: Cell Development

    JOURNAL NAME: CellBio, Vol.3 No.2, June 6, 2014

    ABSTRACT: The primary culture bone marrow (PCBM) cells are often used in different cytological investigations. Here we study the behavior of porcine unstimulated PCBM cells during the cultivation. DNA cytophotometry analyses revealed that immature white blood cells (WBC) such as lymphoblasts, monoblasts and myeloid cells during cultivation process reduced the DNA content in the nuclei. This resulted in displacement of the DNA histogram to the left and increased the content of diploid nuclei. This is a result of cells unblocking which are in G2 cell cycle phase. We also observed diploid pathological cells with accessory or fragmentized nuclei. However, the data with erythroid cells (EC) somehow demonstrate the opposite tendency. During the late stage of cultivation, the number of immature EC with the tetraploid DNA is increased.