TITLE:
Comparison of Protein Expression Profiles of Novel Halomonas smyrnensis AAD6T and Halomonas salina DSMZ 5928T
AUTHORS:
Aydan Salman Dilgimen, Kazim Yalcin Arga, Volker A. Erdmann, Brigitte Wittmann-Liebold, Aziz Akin Denizci, Dilek Kazan
KEYWORDS:
Proteomics, Halomonas salina DSMZ 5928T, Halomonas smyrnensis AAD6T, NEPHGE Technique, Genome
JOURNAL NAME:
Natural Science,
Vol.6 No.9,
May
13,
2014
ABSTRACT:
In this work, the
protein pattern of novel Halomonas
smyrnensis AAD6T was compared to that of Halomonas salina DSMZ5928T, which is the closest species on the basis of 16S rRNA
sequence, to understand how AAD6T differs from type strains. Using high
resolution NEPHEGE technique, the whole cell protein composition patterns
of both Halomonas salina DSMZ5928T and H. smyrnensis AAD6T were mapped. The expressed proteins of the two
microorganisms were mostly located at the acidic side of the gels, at molecular
weight values of 60 to 17 kDa, and at isoelectric points 3.8 to 6.0, where they
share a significant number of common protein spots. Identification and characterization
of protein spots via whole genome sequencing data indicated that these two
microorganisms used similar pathways, especially TCA cycle, for their survival;
in other words, for their energy requirements. On the other hand, the protein
expression differences in AAD6T and H. salina DSMZ 5928T showed that they prefer different
metabolic pathways for lipid biosynthesis and in adaptation to extreme
environments. Thus, we suggested that phylogenetic dissimilarities between
these microorganisms could be related to the protein expression differences; in
other words, metabolic flux differences in AAD6T and H. salina DSMZ 5928T. This is
the first study to explain the dissimilarities of phenotypic characters and
DNA-DNA hybridization between type strain and novel strain AAD6T by
using protein expression differences.