TITLE:
Genetic Diversity and Structure of East African Tall Coconuts in Tanzania Using RAPD Markers
AUTHORS:
Linus I. Masumbuko, Salama Sinje, Alois Kullaya
KEYWORDS:
Cocos nucifera L., RAPD, Genetic Diversity, Nei Genetic Distance
JOURNAL NAME:
Open Journal of Genetics,
Vol.4 No.2,
April
25,
2014
ABSTRACT:
RAPD
markers were used to estimate levels of genetic diversity and structure among
twelve East African Tall (EAT) coconut (Cocos nucifera L.) accessions
that are maintained at Chambezi station. Ten primers were used in the analysis
of 120 accessions. Cluster analysis was performed based on Jaccard’s coefficient
and Nei genetic distances. Further analyses included principal coordinate
analysis (PCA) and finally bootstrap analysis. The cluster analysis revealed
two main clusters. One cluster contained provenances from Lindi and Mtwara
regions (south) and those from Coast and Dar
es Salaam regions (central); the second had
provenances from Tanga region, which is the northern part of the coastal belt.
A similar distribution of provenances was shown by the results of the principal
coordinate analysis, whose first three coordinates explained 56% of the total
variation. The results were able to discriminate between the different
provenances and provide evidence of the different origins for the coconut palms
in the northern and southern parts of coastal coconut-growing belt. The two
major clusters concur well with the history and distribution of coconuts in the
coastal belt of Tanzania.