TITLE:
Differences in age modulates neutrophils function
AUTHORS:
Claudia J. Lagranha, Tatiana C. Alba-Loureiro, Aline Isabel da Silva, Danuta Duarte Bezerra de Lima, Anderson Apolonio Pedroza, Diorginis Ferreira, Tania Pithon-Curi
KEYWORDS:
Testosterone; Neutrophils Function; NADPH-Oxidase Components; Sexual Maturation
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.4 No.1,
February
27,
2014
ABSTRACT:
The sexual maturation in all mammals is the period in
which the quiescent gonads are activated by gonadotropins from anterior
pituitary, increasing the secretion of sexual hormones. Sexual maturation it
is also related with the development of several other body features such as
body mass and maturation of the circulatory, skeletal and hematopoietic
systems. The aim of the present study was to evaluate the function of
neutrophils submitted to in vivo lower and higher concentration of testosterone (sexually immature: 60 days
and sexually mature: 90 days). Using different approaches we evaluated cell
viability and function and gene expression in rat neutrophils from 60 and 90
days-old animals. Neutrophils from 90 days-old rats showed a decrease in
phagocytic and fungicidal capacity, without change in cellular viability.
Additionally, we verified that sexual maturation induced increase in production
of reactive oxygen and nitrogen species (RONS) and also in TNF-α, IL-6 and IL-10 cytokines. In
conclusion, our data suggest that increase in testosterone levels induced
significant alteration in neutrophil function, impairing phagocytic capacity.