TITLE:
Suppression of Fusarium Crown Rot and Increase in Several Free Amino Acids in Mycorrhizal Asparagus
AUTHORS:
Yoh-ichi Matsubara, Tomohiro Okada, Jia Liu
KEYWORDS:
Asparagus Decline; Fusarium proliferatum; GABA; Growth Promotion; Symbiosis
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.2,
January
24,
2014
ABSTRACT:
Disease suppression of Fusarium crown rot
and the changes in free amino acid contents in mycorrhizal asparagus (Asparagus officinalis L., cv. “Welcome”) plants were
investigated. Sixteen weeks after arbuscular mycorrhizal fungus (AMF; Glomus intraradices) inoculation, mycorrhizal plants showed higher dry
weight of shoots than non-mycorrhizal plants, and AMF colonization level in a
root system reached up to 73.3%. Ten weeks after Fusarium proliferatum (Fp; N1-31, SUF1207)
inoculation, control plants showed 100% incidence and high severity in the 2 Fp
isolates. However, AMF plants showed lower severity than non-AMF plants in the
2 Fp isolates. Ten weeks after Fp (N1-31) inoculation, the increase in 7
constituents of amino acids (glutamine, arginine, aspartic acid, alanine,
citrulline, GABA, glycine) in shoots, and 9 in roots (asparagine, arginine,
threonine, serine, glutamine, citrulline, valine, GABA, histidine) occurred in
AMF plants. From these findings, plant growth promotion and suppression of Fusarium crown rot occurred in mycorrhizal asparagus plants, and the disease tolerance
was supposed to be associated with the symbiosis-specific increase in free amino
acids.