TITLE:
Evaluation of Analgesic and Anti-Inflammatory Activities on Ethanolic Extract of Terminalia chebula Fruits in Experimental Animal Models
AUTHORS:
Md. Safkath Ibne Jami, Zakia Sultana, Md. Ershad Ali, Mst. Marium Begum, Md. Mominul Haque
KEYWORDS:
Terminalia chebula; Analgesic; Anti-Inflammatory; Hot Plate; Carrageenan Induced Paw Edema
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.1,
January
13,
2014
ABSTRACT:
The present study was undertaken to evaluate the analgesic and
anti-inflammatory activities of ethanolic extract of Terminalia chebula (commonly known as Haritaki) fruits in
experimental animal models. The study was carried out using Swiss Albino mice (20-25 g) and Long Evans rats (100-150 g) of either sex.
The analgesic activity of Terminalia
chebula was assessed by using hot plate method. For the determination of
analgesic effect, doses of ethanolic extract of Terminalia chebula used in the present study were 250 mg/kg and 500 mg/kg body weight (BW).
Anti-inflammatory effect was analyzed by carrageenan induced paw edema method
with the administration dose of 300 mg/kg BW of
animals. The analysis of experimental data was performed by statistical process
of ANOVA to determine the variability of sample, while Dunnet’s test was
performed for evaluation of comparative analgesic and anti-inflammatory
activity of Terminalia chebula with
control and standard. The animals were divided into four treatment groups of
six animals each and the “Mean ± SEM” is the statistical identifiable value of
the data and P values mg/kg and 500 mg/kg BW doses throughout the observation period in 30 minutes and 60
minutes after treatment, which was comparable to the standard ketorolac and
control group. In carrageenan induced paw edema method, considerable results were found after determining the percentage change in paw volume in extract.
In both cases of analgesic and anti-inflammatory
study, % inhibition of pain and inflammation were evaluated. Comparing with
control, largest inhibition was found in inhibiting inflammation 5
hours after treatment, while the largest inhibition of pain was obtained in 30 minutes and 60 minutes after treatment of doses. The present study suggests that ethanolic extract
of Terminalia chebula fruits has
significant analgesic and anti-inflammatory activities.