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Kewellow, H., Heipieper, H.-J. and Rehm, H.-J. (1989) Protection of bacteria against toxicity of phenol by immobilization in calcium alginate, Applied Micro- biology and Biotechnology, 31, 283-389.

has been cited by the following article:

  • TITLE: Ca-alginate spheres behavior in presence of some solvents and water-solvent mixtures

    AUTHORS: Luis G. Torres, Angelica Velasquez, Marco A. Brito-Arias

    KEYWORDS: Dielectric Constant; Ca-Alginate; Immobilization, Solvents; Spheres; Polarity Index

    JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.2 No.1, February 24, 2011

    ABSTRACT: Immobilization systems more frequently used are calcium alginate spheres. These biocatalysts have many potential applications in the immobilization of enzymes, prokaryotic cells, vegetal and animal cells, algae, organelles and mixtures of these living components. Other applications of immobilized cells imply the use of non aqueous systems. Some bioconversions are carried out in the presence of solvents such as hexane acetone or acetonitrile, or mixtures water-solvents. The aim of this work was to investigate the behaviour of Ca-alginate spheres when put in contact with different solvents (water, diesel, ethanol, methanol, acetone, n-hexane, isopropyl alcohol, THF, acetonitrile, and toluene), or solvent-water mixtures (i.e., ethanol-water), regarding the resistance of the alginate spheres after days of contact. Calcium alginate particles suffered different damages, depending on the solvent they were put in contact. Water did not damaged the Ca-alginate structure with or without Ca present. On the other hand different solvents lost a proportion of volume, i.e., n-hexane (16%), methanol (19%), ethanol (19.5%), toluene (22%), diesel (34%), acetone (765), isopropyl alcohol (80%), THF and acetonitrile (total loss, total destruction). Nor the dielectric constant nor the polarity indexes were capable of explaining the difference on the volume loss or total sphere destruction, except for water-ethanol mixtures.