Prevalence of Malaria Positive Rapid Diagnostic Test and Antimalarial Treatment in Patients with Fevers in the Accident and Emergency Unit of Effia Nkwanta Regional Hospital, Western Region, Ghana


Background: Rapid diagnostic test (RDT) is a simpler, easy to read malaria diagnostic test. It was introduced by the World Health Organization (WHO) to supplement the use of microscopy and can be used alone in areas where microscopy is unavailable. Its introduction was necessary to maintain the WHO test-based treatment protocol for malaria, as dependence of microscopy which is the gold standard is not possible in many areas in Sub-Saharan Africa which lack the wherewithal to run efficient laboratory services. WHO strongly recommends that only patients with parasitological confirmation of malaria should be treated with antimalarial drugs. In this study, the prevalence of malaria positive RDT and antimalarial treatment was evaluated in patients presenting with fevers at the outpatient section of the accident and emergency unit of the Effia Nkwanta Regional Hospital, Ghana. Methodology: This was a retrospective study carried out in the outpatient section of the accident emergency unit of Effia Nkwanta Regional Hospital. The outpatient register was reviewed from October 2014 to March 2015, for patients who came with fever. Data on demographics, malaria RDT status and antimalarial treatment were collected and analyzed. Result: A total of 607 patients with fever had their RDT performed. Of these, 131 (21.58%) were positive for malaria while 467 (78.42%) were negative. Out of the 131 tested positive, 55 represented patients above 12 years and 76 represent children aged 0 to12 years, p = 0.002. Fifty children under the age of 5 years tested positive for malaria whereas 26 were above the age 5 years, p = 0.03. All the 131 positive patients were treated for malaria while 276 out of 476 negative patients were also treated for malaria, p < 0.001. Conclusion: Patients positive for malaria RDT in this study were lower. Children were more positive for malaria than adults, with those under 5 years constituting the majority. This study also shows that indiscriminate malaria prescription is still a problem, a situation that should be tackled immediately, to prevent malaria parasite being resistant to current antimalarial drugs.

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Orish, V. , Ofori-Amoah, J. , Afeke, I. , Jamfaru, I. , Adongo, D. and Amegan-Aho, K. (2016) Prevalence of Malaria Positive Rapid Diagnostic Test and Antimalarial Treatment in Patients with Fevers in the Accident and Emergency Unit of Effia Nkwanta Regional Hospital, Western Region, Ghana. Open Access Library Journal, 3, 1-8. doi: 10.4236/oalib.1103097.

1. Introduction

Malaria remains a serious health problem in Sub-Saharan Africa [1] . Aside the burden of the disease in the population especially in pregnant women and children less than 5 years, malaria has strained the perennial dilapidated health sector in Africa [2] . Diagnostic lapses in the health sector in sub-Saharan African have taken its toll in the diagnosis and treatment of malaria [3] . There are widespread diagnostic bottlenecks being experienced in hospitals and health centers in rural and urban areas in sub-Saharan Africa [4] [5] .

Changes in policy and clinical protocol in the diagnosis and treatment of malaria over the past decade was necessary to overcome the challenges in diagnosis and to accommodate the changing dynamics of malaria burden in the sub region [6] [7] . Presumptive malaria diagnoses based on clinical signs was initially encouraged in the early part of the millennium, especially in children using the integrated management of child hood illness (IMCI) protocol [8] . This was necessary at that time as there was very high burden of malaria among children and prompt treatment was necessary to prevent severe and fatal outcomes [8] . This protocol has long been replaced with a more evidence-based approach that solely depends on parasitological confirmation of malaria before treatment [6] [9] .

The gold standard for malaria diagnosis is microscopy [10] [11] . Though not the most sophisticated laboratory procedure, it is still very much lacking in many hospitals and health centers in developing countries [12] . Many hospitals and health centers are without functional laboratories, the few with these facilities are continuously plagued with incessant breakdowns, stock of out of reagents and lack of adequately trained personnel to deliver reliable microscopy results [13] [14] . The above highlighted challenges are the main reasons why a simpler diagnostic alternative for malaria (rapid diagnostic test) was necessary to help ease up the problems [11] . This is the reason why WHO introduced the malaria rapid diagnostic test (RDT) [11] .

Malaria RDT is an antigen-antibody immunochroma to graphic assay which detects malaria antigen using monoclonal antibodies directed against targeted parasite antigen [15] . It is a very simple test which can be performed anywhere, even in the consulting room, with results within 15 minutes. Rapid diagnostic test can be designed to detect the various species of Plasmodium. However, the RDT in sub-Saharan Africa is specific for Plasmodium falciparum, which is the predominant species in the region. Under normal operational conditions, the RDT is very sensitive, specific and reliable, increasing confidence in test result for both clinician and patient [15] .

In Ghana, evidence-based malaria diagnosis and treatment is being encouraged [9] . With evidence of declining malaria burden, it is very pertinent that test-based malaria diagnoses and treatment be practiced to prevent the non-treatment of other fatal causes of fever especially in children [16] [17] . Since challenges with microscopy are very common in most hospitals and health centers in Ghana, malaria RDT has been deployed to aid in the diagnoses and treatment of malaria. However, despite the relative availability of RDT in most of these facilities, attitude of physicians and other health workers towards the whole idea of test-based treatment is another problem entirely. There are documented evidences that most health workers disregard negative malaria microscopy and prescribe anti-malarial treatment [9] . This same disregard has also been noticed with RDT [9] . The aim of this study was to assess the prevalence of malaria positive RDT and antimalarial treatment among patients with febrile illness seeking care in the accident and emergency unit of the Effia Nkwanta Regional Hospital, Ghana.

2. Materials and Methods

2.1. Study Site

This study was conducted at the Effia-Nkwanta Regional Hospital, the only referral hospital, located south-west of Ghana in the Sekondi-Takoradi metropolis. Sekondi- Takoradi is within the Shama Ahanta east and west municipal region. Effia-Nkwanta Regional Hospital provides services to all other hospitals in the 22 districts and the 13 sub-divisions of the western region.

2.2. Data Collection

This was a retrospective study carried out in the accident and emergency unit of the Effia-Nkwanta Regional Hospital, Ghana. The sampling structure was the outpatient register of the accident and emergency unit of patients who came with fever as one of the presenting complaints. Data was collected from October 2014 to March 2015. Information on demographics, clinical and RDT findings were collected. Also whether Anti-malarial treatment was given was noted. All patients with fever as one of their symptoms, and suspected of malaria, were included in this study. Those in the register but whose RDT results were not traceable were excluded. At the triage area and consulting room of accident and emergency unit of the Hospital, malaria parasite diagnoses were performed using Plasmodium falciparum specific RDT kits (Premier Medical Corporation Ltd, Daman India). The rapid response kit detects P. falciparum antigens and the presence of two lines in the test kit well indicates plasmodiumfalciparum positive.

2.3. Statistical Analysis

All statistical analyses were performed using IBM SPSS Statistics version 21.0 (IBM Corporation, Armonk, NY, USA). Frequency distributions were done for the characteristics of the patient in this study (age, sex, RDT and antimalarial status). These characteristics were further analysed using Pearson χ2 tests. Confidence interval (CI) of 95% was used to measure the strength of the association and p ≤ 0.05 was considered statistically significant.

2.4. Ethical Issues

Permission was granted to carry out this study by the Effia-Nkwanta Regional Hospital authorities.

3. Results

A total of 607 patients with fever had RDT performed during the study period (October 2014 to March 2015). Of these, 307 were males (50.58%) while 300 (49.42%) were females (Table 1). Also as shown in Table 1, 325 (53.54%) of the patients were above 12 years, 71 (11.70%) were from the age of 5 to 12 years and 211 (34.76%) represented children under the age of 5 years Antimalarial treatments were given to 407 (67.05%) of the patients while 200 (32.95%) patients had no treatment. A total of 131 (21.58%) tested positive for malaria, while 476 (78.42%) were negative for malaria (Table 1).

Table 2 stratified the patients into their malaria RDT status. Malaria RDT were posi-

Table 1. Baseline characteristics of 607 patients who attended the accident and emergency unit.

Table 2. Patients’ characteristics stratified by malaria RDT status.

NB: n = number of samples. P values were based on Pearson Chi-Squared test for categorical variables. *n = 76 RDT positive; 206 RTD negative.

tive for 76 children (0 - 12 years) and 55 patients above the age of 12 years, p = 0.002. Further analysis of the 282 children in this study showed positive malaria RDT for 50 younger children (<5 years) and 26 older children (5 - 12 years), p = 0.03 Malaria RDT were positive for 64 males and 67 females (p = 0.67). Anti-malarial treatment was given to the 131 patients positive for malaria RDT and 276 negative for malaria RDT (p ≤ 0.001).

4. Discussion

This study evaluated the prevalence of RDT positive result for malaria and the antimalarial prescription pattern for patients with fever at the accident and emergency of Effia-Nkwanta Regional Hospital, Ghana from October 2014 to March 2015.

There were 607 febrile patients who had RDT done on them. This might not be a true representation of the entire population of patients that came in with fever during the 6 months under retrospective review. It is very likely that there were more patients with fever who came during this period that were not tested because of persistent stock out of the RDT test kits. This stock out is a nagging problem in many health institutions in sub-Saharan Africa, especially Ghana [9] [13] . This could also be due to the fact that malaria was not suspected in some patients with fever, therefore RDT was not done.

Out of the total number of patients who had RDT done on them, almost 22% were RDT positive, while much higher number where RDT negative. This result further shows that malaria is no longer the only major cause of fever in patients as there are evidence of declining burden in the in Ghana [16] [17] .

There were more children who tested positive for malaria with the RDT than adults. This supports the fact that children are still at risk of malaria despite the decreasing burden in the region [18] . Children under 5 were significantly the majority of patients positive for malaria, a finding consistent with studies showing peak malaria prevalence in younger children in stable malaria transmission areas like Ghana [19] - [21] .

All the patients that were positive for malaria had treatment. While this could be a correct practice, it is important to note that RDT could still be positive for weeks even if the patient was successfully treated for malaria. Efforts must be made to investigate for other causes of fever in patients with positive malaria RDT and having prior history for recent antimalarial treatment. It is important to note that over half of the numbers that were negative for malaria were also treated for malaria. This practice of treating malaria without diagnostic confirmation is very common among health care providers [22] [23] . However it has been shown that progressive use of RDT builds the confidence of healthcare providers on its use and subsequently reduces indiscriminate prescription of anti-malarial drugs [24] .

5. Conclusion

This study showed a low prevalence of malaria RDT diagnoses and an indiscriminate antimalarial treatment for patients with fever. This indiscriminate prescription should be curtailed as evidence based medicine is surely the way to go. Test based diagnoses for malaria should be encouraged at all level of health care. RDT test kits should be supplied regularly to ensure best practice be maintained at all time. Health care workers should be trained and re trained on the benefits of test base diagnoses and treatment of malaria. This will go a long way in reducing the financial loss incurred from prescribing antimalarial drugs indiscriminately and also reducing the incidence of drug resistance.


This study cannot vouch for the accuracy in the procedures used by the health care staff in performing and interpreting the malaria RDT. As simple as RDT might be, the manufacturer’s instructions must be strictly adhered to for accurate result to be obtained. So it’s possible that this study might not have captured the true prevalence of malaria in the accident and emergency unit. Thus a more prospective study evaluating the efficiency and accuracy of the methods used will be very useful in estimating the true prevalence of malaria and subsequent antimalarial treatment.


Authors thank the Head of Effia-Nkwanta Regional Hospital and the management for allowing them to take data from their hospital.

Conflict of Interests

The authors have not declared any conflict of interests.

Conflicts of Interest

The authors declare no conflicts of interest.


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