Simple UV Spectrophotometric Assay of Metronidazole


Metronidazole is a derivative of benzimidazole. It is being used widely for the treatment of amoebiasis. An efficient, simple and least time consuming spectrophotometric method for the assay of metronidazole has been developed. Comparison of assay of three different brands of metronidazole has also been available in public medical stores of Karachi, Pakistan. The assay is based on the ultraviolet UV absorbance maxima at about 340 nm wavelength of metronidazole using water as solvent. A sample of drug was dissolved in water to produce a solution containing metronidazole. Similarly, a sample of ground tablets of different brands were dissolved in water and various dilutions were made. The absorbance of sample preparation was measured at 340 nm against the solvent blank and the assay was determined by comparing with the absorbance of available brand. The method can be applied for the routine QC quantitation of metronidazole in tablet formulation actively.

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Naveed, S. and Qamar, F. (2014) Simple UV Spectrophotometric Assay of Metronidazole. Open Access Library Journal, 1, 1-4. doi: 10.4236/oalib.1100615.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Löfmark, S., Edlund, C. and Nord, C.E. (2010) Metronidazole Is Still the Drug of Choice for Treatment of Anaerobic Infections. Clinical Infectious Diseases, 50, S16-S23.
[2] Centers for Disease Control and Prevention (2010) Guidelines for Treatment of Sexually Transmitted Diseases. MMWR Morbidity and Mortality Weekly Report, 47, 8-10.
[3] Jokipii, L. and Jokipii, A.M.M. (1978) Comparison of Four Dosage Schedules in the Treatment of Giardiasis with Metronidazole. Infection, 6, 92-94.
[4] Wright, J.M., Dunn, L.A., Upcroft, P. and Upcroft, J.A. (2003) Efficacy of Antigiardial Drugs. Expert Opinion on Drug Safety, 2, 529-541.
[5] Selgrad, M., Kandulski, A. and Malfertheiner, P. (2009) Helicobacter pylori: Diagnosis and Treatment. Current Opinion in Gastroenterology, 25, 549-556.
[6] Dilshad, H., Naveed, S. and Naqvi, B. (2013) Assay of New Formulations of Isosorbide Mononitrate by Using UV Spectrophotometer: BPJ0000115. World Research Journal of Medicine, 1, 1-2.
[7] Naveed, S., Sultana, N., Arayne, M.S. and Dilshad, H. (2014) A New HPLC Method for the Assay of Levofloxacin and Its Application in Drug-Metal Interaction Studies. Journal of Scientific and Innovative Research, 3, 91-96.
[8] Dilshad, H. and Naveed, S. (2014) Manufacturing of New Formulations of Isosorbide Mononitrate by Dry Granulation Method and Their Comparitive Evaluation with Brands Available in the Market. IRJP, 5, 168-171
[9] Naveed, S., Fatima, Q. and Ghulam, S. (2014) Percentage Assay of Metformin in Different Medium Using UV-Spectrophotometer BPJ0000130. World Research Journal of Organic Chemistry, 2, 2320-5679,
[10] Naveed, S. (2014) Simple UV Spectrophotometric Assay of Atorvastatin API Formulation and Their Comparative Study. Global Journal of Medical Research, 14, 35-38.
[11] Dilshad, H., Naveed, S. and Waheed, N. (2014) Comparitive Study of Four Different Brands of Captopril Available in Karachi. IRJP, 5, 374-377.
[12] Naveed, S. and Qamar, F. (2014) Comparative Study of Different Brands of Alprazolam. Global Journal of Medical Research, 14, 25-28.

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