A HILIC Mechanism Discussion for the Retention of HP-β-CD

Jianqiang Wei¹, Hui Wang^2*, Song Wu³

¹Zhengzhou Tongtai Medical Science & Technology Co., Ltd., Zhengzhou, China.
²Henan Province Health Albert Biomedical Research Co., Ltd, Zhengzhou, China.
³Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

DOI: 10.4236/oalib.1100435                

Abstract

A hydrophilic interaction liquid chromatography (HILIC) method coupled with a refractive index (RI) detector was developed for the determination of hydroxypropyl-β-cyclodextrin (HP-β-CD). This HILIC method used simple acetonitrile/water (80/20, v/v) as the mobile phase to provide sufficient retention for HP-β-CD. The method was found to be specific without interference from the other constituents in pharmaceutics. The method was also validated for linearity and accuracy of the drug sample. The effects of various parameters, such as acetonitrile content, mobile phase pH, and column temperature on HILIC analysis were investigated. Furthermore, the retention mechanism was assessed by the evaluation of the common chromatographic parameters.

Keywords

Hydroxypropyl-β-Cyclodextrin, Quantitative Analysis, Pinocembrin, HILIC

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Lôftsson, T. and Brewster, M.E. (1996) Pharmaceutical Applications of Cyclodextrins. 1. Drug Solubilization and Stabilization. Journal of Pharmaceutical Sciences, 85, 1017-1025. http://dx.doi.org/10.1021/js950534b
[2]	Wong, J.W. and Yuen, K.H. (2001) Improved Oral Bioavailability of Artemisinin through Inclusion Complexation with Beat- and Gama-Cyclodextrins. International Journal of Pharmaceutics, 227, 177-185. http://dx.doi.org/10.1016/S0378-5173(01)00796-7
[3]	Choi, H.G., Kim, D.D., Jun, H.W., Yoo, B.K. and Yong, C.S. (2003) Improvement of Dissolution and Bioavailability of Nitrendipine Byinclusion in Hydroxypropyl β-Cyclodextrin. Drug Development and Industrial Pharmacy, 29, 1085-1094.
[4]	Slain, D., Rogers, P.D., Chapman, S.W. and Cleary, J.D. (2001) Intravenous Itraconazole: A Formulary Focus. Annales Pharmaceutiques Françaises, 35, 720-729.
[5]	Gould, S. and Scott, R.C. (2005) 2-Hydroxypropyl-beta-Cyclodextrin (HP-beta-CD): A Toxicology Review. Food and Chemical Toxicology, 43, 1451-1459. http://dx.doi.org/10.1016/j.fct.2005.03.007
[6]	European Pharmacopoeia. 5th Edition, 1771.
[7]	Ikegami, T., Tomomastu, K., Takubo, H., Horie, K. and Tanaka, N. (2008) Separation Efficiencies in Hydrophilic Interaction Chromatography. Journal of Chromatography A, 1184, 474-503. http://dx.doi.org/10.1016/j.chroma.2008.01.075
[8]	Liu, M., Chen, E. X., Ji, R. and Semin, D. (2008) Stability-Indicating Hydrophilic Interaction Liquid Chromatography Method for Highly Polar and Basic Compounds. Journal of Chromatography A, 1188, 255-263. http://dx.doi.org/10.1016/j.chroma.2008.02.071
[9]	Zhou, T. and Lucy, C.A. (2008) Hydrophilic Interaction Chromatography of Nucleotides and Their Pathway Intermediates on Titania. Journal of Chromatography A, 1187, 87-93. http://dx.doi.org/10.1016/j.chroma.2008.02.027
[10]	Yoshida, T. (2004) Peptide Separation by Hydrophilic-Interaction Chromatography: A Review. Journal of Biochemical and Biophysical Methods, 60, 265-280. http://dx.doi.org/10.1016/j.jbbm.2004.01.006
[11]	Guo, Y. and Huang, A.H. (2003) A HILIC Method for the Analysis of Tromethamine as the Counter Ion in an Investigational Pharmaceutical Salt. Journal of Pharmaceutical and Biomedical Analysis, 31, 1191-1201. http://dx.doi.org/10.1016/S0731-7085(03)00021-9
[12]	Hao, Z.G., Lu, C.Y., Xiao, B., Weng, N.D., Parker, B., Knapp, M. and Ho, C.T. (2007) Separation of Amino Acids, Peptides and Corresponding Amadori Compounds on a Silica Column at Elevated Temperature. Journal of Chromatography A, 1147, 165-171. http://dx.doi.org/10.1016/j.chroma.2007.02.057