Stability of the Antioxidant Activity of Flavonoids after Gamma Irradiation


Food irradiation has the purpose of destroying insects or microorganisms, thereby increasing the safety and shelf life of foods. Flavonoids are ubiquitous plant secondary products with radical scavenger ability. In the present study their antioxidant stability after gamma irradiation was evaluated. The flavonoids showed fast scavenger ability measured with the 2.2-diphenyl-1-picrylhydrazyl radical (DPPH) after high doses of radiation. The low damage caused by irra- diation on antioxidant capacity of the flavonoids shows their potential use in combating chemical oxidation of bio- molecules in irradiated foods.

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A. Vaz, M. Souza, A. Argôlo, A. Melo, M. Oliva, H. Xavier, L. Coelho and M. Correia, "Stability of the Antioxidant Activity of Flavonoids after Gamma Irradiation," Food and Nutrition Sciences, Vol. 2 No. 5, 2011, pp. 490-493. doi: 10.4236/fns.2011.25071.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] E. S. Kempner, “Effects of High-Energy Electrons and Gamma Rays Directly on Protein Molecules,” Journal of Pharmaceutical Sciences, Vol. 90, No. 10, 2001, pp. 1637-1646. doi:10.1002/jps.1114
[2] J. Terao, M. Piskulaand and Q. Yao, “Protective Effect of Epicatechin, Epicatechin Gallate and Quercetin on Lipid Peroxidation in Phospholipid Bilayers,” Archives of Biochemistry and Biophysics, Vol. 308, No. 1, 1994, pp. 278-284. doi:10.1006/abbi.1994.1039
[3] O. I. Aruoma, “Free Radicals, Oxidative Stress, and Anti-Oxidants in Human Health and Disease,” Journal of the American Oil Chemists Society, Vol. 75, No. 2, 1998, pp. 199-212. doi:10.1007/s11746-998-0032-9
[4] B. Winkel-Shirley, “Biosynthesis of Flavonoids and Effects of Stress,” Current Opinion in Plant Biology, Vol. 5, No. 3, 2002, pp. 218-223. doi:10.1016/S1369-5266(02)00256-X
[5] M. Garcia-Alonso, G. Rimbach, M. Sasai, M. Nakahara, S. Matsugo, Y. Uchida, J. C. Rivas-Gonzalo and S de Pascual-Teresa, “Electron Spin Resonance Spectroscopy Studies on the Free Radical Scavenging Activity of Wine Anthocyanins and Pyranoanthocyanins,” Molecular Nutrition & Food Research, Vol. 49, No. 12, 2005, pp. 1112-1119. doi:10.1002/mnfr.200500100
[6] F. Breitfellner, S. Solar and G. Sontag, “Effect of Gamma Irradiation on Flavonoids in Strawberries,” European Food Research & Technology, Vol. 215, No. 1, 2002, pp. 28-31. doi:10.1007/s00217-002-0536-8
[7] N. Orsoli?, V. Benkovi?, A. Horvat-Knezevi?, N. Kopjar, I. Kosalec, M. Bakmaz, Z. Mihaljevi?, K. Bendelja and I. Basi?, “Assessment by Survival Analysis of the Radioprotective Properties of Propolis and its Polyphenolic Compounds,” Biological & Pharmaceutical Bulletin, Vol. 30, No. 5, 2007, pp. 946-951. doi:10.1248/bpb.30.946
[8] V. Benkovi?, N. Orsoli?, A. H. Knezevi?, S. Rami?, D. Diki?, I. Basi? and N. Kopjar, “Evaluation of the Radioprotective Effects of Propolis and Flavonoids in Gamma-Irradiated Mice: The Alkaline Comet Assay Study,” Biological & Pharmaceutical Bulletin, Vol. 31, No. 1, 2008, pp. 167-172. doi:10.1248/bpb.31.167
[9] V. Benkovi?, A. H. Knezevi?, D. Diki?, D. Lisici?, N. Orsoli?, I. Basi? and N. Kopjar, “Radioprotective Effects of Quercetin and Ethanolic Extract of Propolis in Gamma-Irradiated Mice,” Archives of Industrial Hygiene & Toxicology, Vol. 60, No. 2, 2009, pp. 129-138. doi:10.2478/10004-1254-60-2009-1908
[10] Y. Hanasaki, S. Ogawa and S. Fukui, “The Correlation between Active Oxygens Scavenging and Antioxidative Effects of Flavonoids,” Free Radical Biology & Medicine, Vol. 16, No. 6, 1994, pp. 845-850. doi:10.1016/0891-5849(94)90202-X
[11] D. Kozlowski, P. Marsal, M. Steel, R. Mokrini, J. L. Duroux, R. Lazzaroni and P. Trouillasa, “Theoretical Investigation of the Formation of a New Series of Antioxidant Depsides from the Radiolysis of Flavonoid Compounds,” Radiation Research, Vol. 168, No. 2, 2007, pp. 243-252. doi:10.1667/RR0824.1
[12] W. Brand-Williams, M. E. Cuvelier and C. Berset, “Use of a Free Radical Method to Evaluate Antioxidant Activity,” LWT-Food Science and Technology, Vol. 28, No. 1, 1995, pp. 25-30.
[13] C. Soler-Rivas, J. C. Esp?n and H. J. Wichers, “An Easy and Fast Test to Compare Total Free Radical Scavenger Capacity of Foodstuffs,” Phytochemistry Analysis, Vol. 11, No. 5, 2000, pp. 1-9. doi:10.1002/1099-1565(200009/10)11:5<330::AID-PCA534>3.0.CO;2-G
[14] A. Moure, D. Franco, J. Sineiro, H. Dominguez, M. J. Nú?es and J. M. Lema, “Antioxidant Activity of Extracts from Gevuina Avellana and Rosa Rubiginosa Defatted Seeds,” Food Research International, Vol. 34, No. 2-3, 2001, pp. 103-109. doi:10.1016/S0963-9969(00)00136-8
[15] P. Swatsitang, G. Tucker, K. Robards and D. Jardine, “Isolation and Identification of Phenolic Compounds in Citrus Sinensis,” Analytica Chimica Acta, Vol. 417, No. 2, 2000, pp. 231-240. doi:10.1016/S0003-2670(00)00937-5
[16] D. Ferreira and D. Slade, “Oligomeric Proanthocyanidins: Naturally Occurring O-Heterocycles,” Natural Product Reports, Vol. 19, No. 5, 2002, pp. 517-541. doi:10.1039/b008741f
[17] C. Zhao, Y. Shi, W. Wang, W. Lin, B. Fan, Z. Jia, S. Yao and R. Zheng, “Fast Repair Activities of Quercetin and Rutin toward dGMP Hydroxyl Radical Adducts,” Radiation Physics and Chemistry, Vol. 63, No. 2, 2002, pp. 137-142. doi:10.1016/S0969-806X(01)00224-9
[18] A. Marfak, P. Trouillas, D. P. Allais, Y. Champavier, C. A. Calliste and J. L. Duroux, “Radiolysis of Kaempferol in Water/Methanol Mixtures. Evaluation of Antioxidant Activity of Kaempferol and Products Formed,” Journal of Agricultural and Food Chemistry, Vol. 51, No. 5, 2003, pp. 1270-1277. doi:10.1021/jf020836g
[19] A. S. Pannala, T. S. Chan, P. J. O’Brien and C. A. Rice-Evans, “Flavonoid B-Ring Chemistry and Antioxidant Activity: Fast Reaction Kinetics,” Biochemical and Biophysical Research Communications, Vol. 282, No. 5, 2001, pp. 1161-1168. doi:10.1006/bbrc.2001.4705

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