Induced Production of Exoglucanase, and β-Glucosidase from Fungal Co-Culture of T. viride and G. lucidum


In the present study, a co-culture technique was adopted with an aim to investigate a hyper production of exoglucanase, and β-glucosidase using cheap and easily available agro-industrial residue corn stover as growth supporting substrate. Various physio-chemical and nutritional variables were optimized using classical and completely randomized designs for induced production of exoglucanase, and β-glucosidase from the co-culture of Trichoderma viride and Ganoderma lucidum in solid state fermentation (SSF). Analysis profile showed that when the conditions of the SSF medium containing 15 g corn stover substrate (50% w/w moisture) inoculated with 6 mL of inoculum were optimal, the maximum productions of exoglucanase (485 ± 6.5 U/mL) and β-glucosidase (255 ± 3.3 U/mL) were recorded after 5 days of incubation at pH 6 and 35°C.

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Shahzadi, T. , Anwar, Z. , Iqbal, Z. , Anjum, A. , Aqil, T. ,  , B. , Afzal, A. , Kamran, M. , Mehmood, S. and Irshad, M. (2014) Induced Production of Exoglucanase, and β-Glucosidase from Fungal Co-Culture of T. viride and G. lucidum. Advances in Bioscience and Biotechnology, 5, 426-433. doi: 10.4236/abb.2014.55051.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Iqbal, H.M.N., Asgher, M., Ahmed, I. and Hussain, S. (2010) Media Optimization for Hyper-Production of Carboxymethyl Cellulase Using Proximally Analyzed Agro-Industrial Residue with Trichoderma harzianum under SSF. IJAVMS, 4, 47-55.
[2] Irshad, M., Anwar, Z. and Afroz, A. (2012) Characterization of Exo 1, 4-β Glucanase Produced from Tricoderma viridi through Solid-State Bio-Processing of Orange Peel Waste. Advances in Bioscience and Biotechnology, 3, 580-584.
[3] Iqbal, H.M.N., Ahmed, I., Zia, M.A. and Irfan, M. (2011) Purification and Characterization of the Kinetic Parameters of Cellulase Produced from Wheat Straw by Trichoderma viride under SSF and Its Detergent Compatibility. Advances in Bioscience and Biotechnology, 2, 149-156.
[4] Iqbal, H.M.N., Asgher, M. and Bhatti, H.N. (2011) Optimization of Physical and Nutritional Factors for Synthesis of Lignin Degrading Enzymes by a Novel Strain of Trametes versicolor. BioResources, 6, 1273-87.
[5] Irshad, M., Anwar, Z., But, H.I., Afroz, A., Ikram, N. and Rashid, U. (2013) The Industrial Applicability of Purified Cellulase Complex Indigenously Produced by Trichoderma viride through Solid-State Bio-Processing of Agro-Industrial and Municipal Paper Wastes. BioResources, 8, 145-157.
[6] Iqbal, H.M.N., Kyazze, G. and Keshavarz, T. (2013) Advances in Valorization of Lignocellulosic Materials by Bio-Technology: An Overview. BioResources, 8, 3157-3176.
[7] Quiroz-Castañeda, R.E., Balcázar-López, E., Dantán-González, E., Martinez, A., Folch-Mallol, J. and Anaya, C.M. (2009) Characterization of Cellulolytic Activities of Bjerkandera adusta and Pycnoporus sanguineus on Solid Wheat Straw Medium. Electronic Journal of Biotechnology, 12, 5-6.
[8] Iqbal, H.M.N., Kamal, S., Ahmed, I. and Naveed, M.T. (2012) Enhanced Bio-Catalytic and Tolerance Properties of an Indigenous Cellulase through Xerogel Immobilization. Advances in Bioscience and Biotechnology, 3, 308-313.
[9] Yano, S., Ozaki, H., Matsuo, S., Ito, M., Wakayama, M. and Takagi, K. (2012) Production, Purification and Characterization of D-Aspartate Oxidase from the Fungus Trichoderma harzianum SKW-36. Advances in Bioscience and Biotechnology, 3, 7-13.
[10] Deshpande, M.V., Eriksson, K.E. and Göran Pettersson, L. (1984) An Assay for Selective Determination of Exo-1, 4,-β-Glucanases in a Mixture of Cellulolytic Enzymes. Analytical Biochemistry, 138, 481-487.
[11] Gielkens, M.M.C., Dekkers, E., Visser, J. and De-Graaff, L.H. (1999) Two Cellobiohydrolase-Encoding Genes from Aspergillus niger Require D-Xylose and the Xylanolytic Transcriptional Activator XlnR for Their Expression. Applied and Environmental Microbiology, 65, 4340-4545.
[12] Chandra, M., Kalra, A. and Sharma, P.K. (2010) Optimization of Cellulases Production by Trichoderma citrinoviride on Marc of Artemisia annua and Its Application for Bioconversion Process. Biomass and Bioenergy, 34, 805-811.
[13] Pushalkar, S., Rao, K.K. and Menon, K. (1995) Production of β-Glucosidase by Aspergillus terrus. Current Microbiology, 30, 255-258.
[14] Omojasola, P.F. and Jilani, O.P. (2009) Cellulase Production by Trichoderma longi, Aspergillus niger and Saccharomyces cerevisae Cultured on Plantain Peel. Research in Microbiology, 4, 67-74.
[15] Mikiashvili, N., Elisashvili, V., Wasser, S. and Nevo, E. (2005) Carbon and Nitrogen Sources Influence the Ligninolytic Enzyme Activity of Trametes versicolor. Biotechnology Letters, 27, 955-959.

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