Repeated application of autologous bone marrow-derived stem cell therapy in patients with severe Buerger’s disease
Zoltan Boda, K. Razso, M. Szarvas, Zs. Olah, P. Ilonczai, Z. Vereb, E. Rajnavolgyi
DOI: 10.4236/scd.2011.11002   PDF    HTML     4,532 Downloads   11,181 Views   Citations


Stem cell therapy (SCT) is a promising and prospective approach in the treatment of patients with severe peripheral arterial disorders, primarily with Buerger’s disease. However, very little is known about the duration of the effect of SCT, and to our best knowledge no data are available on the efficacy and safety of repeated SCT in patients with Buerger’s disease. Here we report on two patients with severe Buerger’s disease, who received repeated autologous bone marrow-derived stem cell therapy. Our results show that a second SCT, applied to a previously treated leg 30 or 36 months after the first treatment was efficient in both cases. After twelve months, the clinical state of the repeatedly treated lower limb improved spectacularly and non-healing ulcers healed more rapidly than after the first SCT. No severe adverse events were detected. Thus repeated SCT offers a safe and efficient treatment option for relapsing patients at the advanced stage of Buerger’s disease.

Share and Cite:

Boda, Z. , Razso, K. , Szarvas, M. , Olah, Z. , Ilonczai, P. , Vereb, Z. and Rajnavolgyi, E. (2011) Repeated application of autologous bone marrow-derived stem cell therapy in patients with severe Buerger’s disease. Stem Cell Discovery, 1, 16-19. doi: 10.4236/scd.2011.11002.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Ishida, A., Ohya, Y., Sakuda, H., Ohshiro, K., Higashiuesato, Y., Nakaema, M., Matsubara, M., Yakabi, S., Kakihara, A., Ueda, M., Miyagi, C., Yamane, N., Koja, K., Komori, K. and Takashita, S. (2005) Autologous peripheral blood mononuclear cell implantation for patients with peripheral arterial disease improves limb ischemia, Circulation Journal, 69, 1260-1265. doi:10.1253/circj.69.1260
[2] Irrera, G., Console, G., Martino, M., Puci, G., Morabito, F., Callea, I., Pontari, A., Marcuccio, D., Cuzzola, M., Dattola, A., Condemi, A., Messina G. and Iacopino, A.P. (2005) Therapeutic angiogenesis after infusion of bone marrow mononuclear cells for Buerger’s disease: Case report, Bone Marrow Transplantation, 36, S92.
[3] Miyamoto, K., Nishigami, K., Nagaya, N., Akutsu, K., Chiku, M., Kamei, M., Toshihiro, S., Shigeki, M., Masahiro, H., Ryoichi, T., Takeshi, N., Hiroshi, N. and Satoshi, T. (2006) Unblinded pilot study of autologous transplantation of bone marrow mononuclear cells in patients with thromboangiitis obliterans, Circulation, 114, 2679-2684. doi:10.1161/CIRCULATIONAHA.106.644203
[4] Durdu, S., Akar, A.R., Arat, M., Sancak, T., Eren, N.T. and Ozyurda, U. (2006) Autologous bone-marrow mononuclear cell implantation for patients with Rutherford grade II-III thromboangiitis obliterans, Journal of Vascular Surgery, 44, 732-739. doi:10.1016/j.jvs.2006.06.023
[5] Kim, D.I., Kim, M.J., Joh, Shin, J.H., Do, S.W., Moon, Y.S., Kim, J.Y., Lim, N.R., Kim, J.E., Eo, H.S., Kim, A.K., Cho, S.W., Yang, S.H., Park C.J. and Shim J.S. (2006) Angiogenesis facilitated by autologous whole bone marrow stem cell transplantation for Buerger's disease, Stem Cells, 24, 1194-1200. doi:10.1634/stemcells.2005-0349
[6] Kim, S.W., Han, H., Chae, G.T., Lee, S.H., Bo, J.H., Yoon, S., Lee, Y.S., Lee, K.S., Park H.K. and Kang, K.S. (2006) Successful stem cell therapy using umbilical cord blood-derived multipotent stem cells for Buerger's disease and ischemic limb disease animal model, Stem Cells, 24, 1620-1626. doi:10.1634/stemcells.2005-0365
[7] Kajiguchi, M., Kondo, T., Izawa, H., Kobayashi, M., Yamamoto, K., Shintani, S., Numaguchi, Y., Naoe, T., Takamatsu, T., Komori K., and Murohara, T. (2007) Safety and efficacy of autologous progenitor cell transplantation for therapeutic angiogenesis in patients with critical limb ischemia, Circulation Journal, 71, 196-201. doi:10.1253/circj.71.196
[8] Saito, Y., Sasaki, K., Katsuda, Y., Murohara, T., Takeshita, Y., Okazaki, T., Arima, K., Katsuki, Y., Shintani, S., Shimada, T., Akashi, H., Ikeda H., and Imaizumi, T. (2007) Effect of autologous bone-marrow cell transplantation on ischemic ulcer in patients with Buerger’s disease”, Circulation Journal, 71, 1187-92. doi:10.1253/circj.71.1187
[9] Boda, Z., Udvardy, M., Razso, K., Farkas, K., Toth, J., Jambor, L., Olah, Zs, Ilonczai, P., Szarvas, M., Kappelmayer, J., Veréb Z., and Rajnavolgyi, E. (2009) Stem cell therapy: A promising and prospective approach in the treatment of patients with severe Buerger’s disease, Clinical and Applied Thrombosis/Hemostasis, 15, 552-560. doi:10.1177/1076029608319882
[10] Wang, W., Liu, C., Qiao, T., Liu C., and Huang, D. (2010) Clinical efficacy of autologous bone marrow mononuclear cell transplantation in treating lower limb thromboangiitis obliterans, Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi, 24, 420-423.
[11] Motukuru, V., Suresh, K.R., Vivekanand, V., Raj, S. and Girija, K.R. (2008) Therapeutic angiogenesis in Buerger’s disease (thromboangiitis obliterans) patients with critical limb ischemia by autologous transplantation of bone marrow mononuclear cells, Journal of Vascular Surgery, 48, 53S-60S. doi:10.1016/j.jvs.2008.09.005
[12] Matoba, S., Tatsumi, T., Murohara, T., Imaizumi, T., Katsuda, Y., Ito, M., Saito, Y., Uemura, S., Suzuki, H., Fukumoto, S., Yamamoto, Y., Onodera, R., Teramukai, S., Fukushima, M., Matsubara H. and TACT Follow-up Study Investigators (2008) Long-term clinical outcome after intramuscular implantation of bone marrow mononuclear cells (Therapeutic Angiogenesis by Cell Transplantation [TACT] trial) in patients with chronic limb ischemia, American Heart Journal, 156, 1010-1018. doi:10.1016/j.ahj.2008.06.025
[13] Kakihana, A., Ishida, A., Miyagi, M., Ishiki, T., Okumura, K., Kamiyama, T., Ohya, Y. and Takishita, S. (2009) Improvement of cardiac function after granulocyte-colony stimulating factor-mobilized peripheral blood mononuclear cell implantation in a patient with non-ischemic dilated cardiomyopathy associated with thromboangiitis obliterans, Internal Medicine, 48, 1003-1007. doi:10.2169/internalmedicine.48.2117
[14] De Vriese, A.S., Billiet, J., Van Droogenbroeck J., Ghekiere J. and De Letter J.A. (2008) Autologous transplantation of bone marrow mononuclear cells for limb ischemia in a caucasian population with atherosclerosis obliterans, Journal Internal Medicine, 263, 395-403. doi:10.1111/j.1365-2796.2007.01899.x
[15] Fadini, G.P., Agostini, C. and Avogaro, A. (2010) Autologous stem cell therapy for peripheral arterial disease meta-analysis and systematic review of the literature, Atherosclerosis, 209, 10-17. doi:10.1016/j.atherosclerosis.2009.08.033
[16] Lawall, H., Bramlage, P. and Amann, B. (2010) Stem cell and progenitor cell therapy in peripheral artery disease, Thrombosis Haemostasis, 103, 696-709. doi:10.1160/TH09-10-0688
[17] Awad, O., Dedkovm, E.I. and Jiao, C. (2006) Differential healing activities of CD34+ and CD14+ endothelial cell progenitors, Arteriosclerosis Thrombosis and Vascular Biology, 26, 758-764. doi:10.1161/01.ATV.0000203513.29227.6f
[18] Zampetaki, A., Kirton, J.P. and Xu, Q. (2008) Vascular repair by endothelial progenitor cells, Cardiovascular Research, 78, 413-421. doi:10.1093/cvr/cvn081

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.