Relation between serum progranulin, inflammatory markers and visceral fat in childhood obesity


Aim: To study serum progranulin (PGRN) level in children with obesity and its relationship to inflamamatory markers and visceral fat. Methods: Fifty obese children and 50 controls aged 10-18 years were recruited. Demographic, anthropometric and biochemical features were collected according to a standard protocol. Serum progranulin levels, serum IL-6 and hsCRP were measured using ELISA. Insulin resistance was calculated by the homeostasis model (HOMA-IR) using the following formula: HOMA-IR = fasting insulin (mU/L) × fasting glucose (mmoL/L)/ 22.5. The maximum visceral fat thickness (VFT) and the minimum subcutaneous fat thickness (SFT) were measured by ultrasonography. Results: In the obese group, a significant increase was found in serum PGRN (48.87 ± 42.33 ng/mL) compared to control group (30.18 ± 23.82 ng/mL). Progranulin correlated significantly with VFT (r = 0.475), IL6 (r = 0.368), Insulin(r = 0.440) and HOMA-IR (r = 0.379). The mean serum progranulin in the high tertile VFT group was significantly higher than those in the low tertile and middle tertile groups (P = 0.030 and P = 0.039 respectively). VFT was highly positively correlated to progranulin, SFT, IL6, insulin, HOMA-IR and hsCRP (P = 0.001, 0.000, 0.001, 0.001, 0.003 and 0.003). However, the correlation coefficient between SFT and progranulin was insignificant. Summary: we demonstrated for the first time that serum PGRN concentrations increased in Egyptian obese children. The concentrations of serum PGRN correlated closely with visceral fat and IL6. Conclusion: PGRN may contribute to the pathogenesis of chronic inflammation in obesity. It could be a novel marker of visceral fat in obesity. Thus PGRN could be a potential therapeutic target for management of chronic inflammation in obesity.

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Ismail, N. , Ragab, S. , Abd El Baky, A. , Hamed, M. and Ayoub, D. (2013) Relation between serum progranulin, inflammatory markers and visceral fat in childhood obesity. Advances in Bioscience and Biotechnology, 4, 1030-1038. doi: 10.4236/abb.2013.412138.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] G Ogden, C.L., Carroll, M.D., Kit, B.K. and Flegal, K.M. (2012) Prevalence of obesity and trends in body mass index among US children and adolescents, 1999-2010. Journal of the American Medical Association, 307, 483-490.
[2] Mariusz, S. , Rafal, N.W., Marek, P., et al. (2012) Serum concentrations of adiponectin, leptin, resistin, ghrelin and insulin and their association with obesity indices in obese normo- and hypertensive patients—Pilot study. Archives of Medical Science, 8, 431-436.
[3] Recio-Rodriguez, J.I., Gomez-Marcos, M.A., Patino-Alonso, M.C., Agudo-Conde, C., Rodriguez-Sanchez, E., Garcia-Ortiz, L. and Vasorisk, G. (2012) Abdominal obesity vs general obesity for identifying arterial stiffness, subclinical atherosclerosis and wave reflection in healthy, diabetics and hypertensive. BMC Cardiovascular Disorders, 12, 3-11.
[4] Zúñiga, L.A., Shen, W.J. and Joyce-Shaikh, B., et al. (2010) IL-17regulates adipogenesis, glucose homeostasis, and obesity. Journal of Immunology, 85, 6947-6959.
[5] Fain, J.N. (2006) Release of interleukins and other inflammatory cytokines by human adipose tissue is enhanced in obesity and primarily due to the nonfat cells. Vitamins & Hormones, 74, 443-477.
[6] Blüher, M. (2008) the inflammatory process of adipose tissue. Pediatric Endocrinology Reviews, 6, 24-31.
[7] Ye, J.P. (2009) Emerging role of adipose tissue hypoxia in obesity and insulin resistance. International Journal of Obesity, 33, 54-66.
[8] Daniel, E.D., He, Z. and Bateman, A. (2003) Progranulin (acrogranin/PC cell-derived growth factor/granulin-epithelin precursor) is expressed in the placenta, epidermis, microvasculature, and brain during murine development. Developmental Dynamics, 227, 593-599.
[9] He, Z. and Bateman, A. (2003) Progranulin (granulinepithelin precursor, PC-cell-derived growth factor, acrogranin) mediates tissue repair and tumorigenesis. Journal of Molecular Medicine, 81, 600-612.
[10] Guerra, R.R., Kriazhev, L., Hernandez-Blazquez, F.J. and Bateman, A. (2007) Progranulin is a stress-response factor in fibroblasts subjected to hypoxia and acidosis. Growth Factors, 25, 280-285.
[11] Egyptian growth curves (2009) Diabetes endocrine metabolism pediatric unit Cairo university children’s hospital.
[12] Ismail, N.A., Ragab, S., Abd El Dayem, S.A., Abd ElBaky, A., et al. (2012) Fetuin-A levels in obesity: Differences in relation to metabolic syndrome and correlation with clinical and laboratory variables. Archives of Medical Science, 8, 826-833.
[13] Deurenberg, P., Weststrate, J.A. and Seidell, J.C. (1991) Body mass index as a measure of body fatness: Age- and sex-specific prediction formulas. The British Journal of Nutrition, 65, 105-114.
[14] Wafaa, M.A., Ragab, R., Ismail, N.A., Yasser, Elhosary, A., et al. (2012) Frequency of non-alcoholic fatty liver disease in overweight/obese children and adults: Clinical, sonographic picture and biochemical assessment. Journal of Genetic Engineering and Biotechnology, 10, 221-227.
[15] Kim, S.K., Kim, H.J., Hur, K.Y., et al. (2004) Visceral fat thickness measured by ultrasonography can estimate not only visceral obesity but also risks of cardiovascular and metabolic Diseases. American Journal of Clinical Nutrition, 79, 593-599.
[16] Toshiya, M., Ayako, M., Kohtaro, M., et al. (2012) Progranulin is a key adipokine mediating high fat diet-induced insulin resistance and obesity through IL-6 in adipose tissue. Cell Metabolism, 15, 38-50.
[17] Tönjes, A., Fasshauer, M., Kratzsch, J. and Stumvoll, M., Blüher, M. (2010) Adipokine pattern in subjects with impaired fasting glucose and impaired glucose tolerance in comparison to normal glucose tolerance and diabetes. PLoS ONE, 5, e13911.
[18] Fujioka, S., Matsuzawa, Y., Tokunaga, K. and Tarui, S. (1987) Contribution of intra-abdominal fat accumulation to the impairment of glucose and lipid metabolism in human obesity. Metabolism, 36, 54-59.
[19] Ribeiro-Filho, F.F., Faria, A.N., Kohlmann Jr., O., et al. (2001) Ultrasonography for the evaluation of visceral fat and cardiovascular risk. Hypertension, 38, 713-717.
[20] Howard, J.K. and Flier, J.S. (2006) Attenuation of leptin and insulin signaling by SOCS proteins. Trends in Endocrinology & Metabolism, 17, 365-371.
[21] Rotter, V., Nagaev, I. and Smith, U. (2003) Interleukin-6 (IL-6) induces insulin resistance in 3T3-L1 adipocytes and is, like IL-8 and tumor necrosis factor-a, overexpressed in human fat cells from insulin-resistant subjects. Journal of Biological Chemistry, 278, 45777-45784.
[22] Blüher, M. (2013) Adipose tissue dysfunction contributes to obesity related metabolic diseases. Best Practice & Research Clinical Endocrinology & Metabolism, 27, 163-177.
[23] Van Harmelen, V., Röhrig, K. and Hauner, H. (2004) Comparison of proliferation and differentiation capacity of human adipocyte. Metabolism, 53, 632-637.
[24] Gealekman, O., Guseva, N., Hartigan, C., et al. (2011) Depot-specific differences and insufficient subcutaneous adipose tissue angiogenesis in human obesity. Circulation, 123, 186-194.

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