Screening of Class 1 and Class 2 Integrons in Clinical Isolates of Pseudomonas aeruginosa Collected from Seven Hospitals in Turkey: A Multicenter Study
Aysegul Copur Cicek, Aysegul Saral, Azer Ozad Duzgun, Zeynep Cizmeci, Tuba Kayman, Pervin Ozlem Balci, Tuba Dal, Mehmet Firat, Yelda Yazici, Metin Sancaktar, Osman Birol Ozgumus, Cemal Sandalli
Clinic of Infectious Diseases and Clinical Microbiology, Private OSM Ortadogu Hospital, Sanliurfa, Turkey.
Clinical Microbiology Laboratory, Ahi Evren Chest and Cardiovascular Surgery Research and Training Hospital, Trabzon, Turkey.
Clinical Microbiology Laboratory, Akcaabat Hackali Baba General State Hospital, Trabzon, Turkey.
Clinical Microbiology Laboratory, General State Hospital, Tokat, Turkey.
Clinical Microbiology Laboratory, Kecioren Research and Training Hospital, Ankara, Turkey.
Clinical Microbiology Laboratory, Research and Training Hospital, Kayseri, Turkey.
Department of Biology, Faculty of Arts and Sciences, Artvin Coruh University, Artvin, Turkey.
Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdogan University, Rize, Turkey.
Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Gümü?hane University, Gumushane, Turkey.
Department of Medical Microbiology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey.
Department of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey.
DOI: 10.4236/ojmm.2013.34034   PDF    HTML     4,021 Downloads   6,795 Views   Citations


Pseudomonas aeruginosa is one of the leading nosocomial pathogens worldwide, and their infections are difficult to treat due to acquired resistance to many antibiotics. This study aimed to detect class 1 and 2 integrons and antibiotic susceptibility of clinical isolates of P. aeruginosa. Two hundred and five P. aeruginosa strains were collected from the seven general state hospitals in Turkey. They were characterized by antimicrobial susceptibility testing, screened for class 1 and class 2 integrons, and evaluated for the association between antibiotic resistance phenotypes and the presence of integrons. intI gene was amplified in 10 isolates (4.87%) by PCR and in seven isolates of them (70%) were found different gene cassettes. The aadA gene integrated into the class 1 integrons was most frequently found and it was followed by aac genes and blaOXA family genes. Sequence analysis of variable regions of the class 1 integrons showed five gene cassette arrays; aadA1(99%), aac(3)-Id(82%)-orf-aac(3”)-Ia(99%), aac(3)-Ie(83%)-blaoxa10(100%)- aadA1 (100%), aadA6(99%, 100%), aac(6’)-I(97%)-orf-aadA2(99%). No class 2 integron was detected. This study is the first multicenter study for class 1 integrons and it indicates the low rate of presence of class 1 gene cassette in P. aeruginosa.

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A. Cicek, A. Saral, A. Duzgun, Z. Cizmeci, T. Kayman, P. Balci, T. Dal, M. Firat, Y. Yazici, M. Sancaktar, O. Ozgumus and C. Sandalli, "Screening of Class 1 and Class 2 Integrons in Clinical Isolates of Pseudomonas aeruginosa Collected from Seven Hospitals in Turkey: A Multicenter Study," Open Journal of Medical Microbiology, Vol. 3 No. 4, 2013, pp. 227-233. doi: 10.4236/ojmm.2013.34034.

Conflicts of Interest

The authors declare no conflicts of interest.


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