Evaluation of the Antioxidant Activity of Ethanolic Extracts of Some Varieties of Onion


The content of polyphenolic in commercial onions has been determined. The antioxidant activity of their ethanolic extracts, as well as their effects on the oxidation of edible corn oil during accelerated ageing, was studied. Maceration of taxonomically identified commercial vegetable samples, previously peeled and thinly sliced, was carried out at ambient temperature, out of direct light, with occasional agitation and ultrasound, employing 95% ethyl alcohol as the extraction solvent, allowing them to stand for 7 days. The total polyphenolic contents were determined on the filtrated extracts using the Folin-Ciocalteau method. The antioxidant activity was evaluated on emulsions of ethanolic extracts of onion prepared in edible commercial corn oil, using sorbitan monooleate as emulsifying agent. The peroxide values were analyzed using the iodometric method; oxidation induction times were obtained from the peroxide evolution graphs, using the tangent method. Oil samples emulsified with ethanolic onion extracts showed an extension of the induction period. A 7 day ageing study at 45℃ was additionally performed to determine the conjugated dienes on pure commercial corn oil and its emulsions by visible spectrophotometry. The spectral analysis showed an increase of the measured absorbances in oil samples without additives and no change for the oils emulsified with onion extract. An increasing of diene values was observed for corn oil without additives during ageing; no changes in the value were observed in oils emulsified with onion extracts.

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M. Gutiérrez, P. Rocca, E. Seta and F. Reina, "Evaluation of the Antioxidant Activity of Ethanolic Extracts of Some Varieties of Onion," Food and Nutrition Sciences, Vol. 4 No. 12, 2013, pp. 1260-1265. doi: 10.4236/fns.2013.412161.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] R. H. Liu, “Health Benefits of Fruit and Vegetables Are from Additive and Synergistic Combinations of Phytochemicals,” American Journal of Clinical Nutrition, Vol. 78, No. 3, pp. 517S-520S.
[2] G. Block and L. Langseth, “Antioxidant Vitamins and Disease Prevention,” Food Technology, 1994, pp. 80-84.
[3] M. G. L. Hertog, E. J. M. Feskens, P. C. H. Hollman, M. B. Katan and D. Kromhout, “Dietary Antioxidant Flavonoids and Risk of Coronary Heart Disease, The Zutphen Elderly Study,” The Lancet, Vol. 342, No. 8878, 1993, pp. 1007-1011.
[4] J. Pokorny, N. Yanishlieva and M. Gordon, “Antioxidants in Food. Practical Applications,” Woodhead Publishing Limited, Abington Hall, Abington, 2001.
[5] G. Block, “A Role for Antioxidants in Reducing Cancer Risk,” Nutrition Review, Vol. 50, No. 7, 1992, pp. 207213.
[6] G. Shui and L. Leong, “Residue from Star Fruit as Valuable Source for Functional Food Ingredients and Antioxidant Nutraceuticals,” Food Chemistry, Vol. 97, No. 2, 2006, pp. 277-284.
[7] P. Abuja, M. Murkovic and W. Pfanhauser, “Antioxidant and Prooxidant Activities of Eldeberry (Sambucus nigra) Extract in Low Density Lipoprotein Oxidation,” Journal of Agricultural and Food Chemistry, Vol. 46, No. 10, 1998, pp. 4091-4096. http://dx.doi.org/10.1021/jf980296g
[8] H. Wang, Muraleedharan, M. G. Nair, G. M. Strasburg, Y. Chang, A. M. Booren, I. J. Gray and D. L. DeWitt, “Antioxidant and Antiinflammatory Activities of Anthocyanins and Their Aglycon, Cyanidin, from Tart Cherries,” Journal of Natural Products, Vol. 62, No. 2, 1999, pp. 294-296.
[9] M. Saleh, F. A. Hashem and K. W. Glombitza, “Study of Citrus Taitensis and Radical Scavenger Activity of the Flavonoids Isolated,” Food Chemistry, Vol. 63, No. 3, 1998, pp. 397-400.
[10] H. M. Dawes and J. B. Keene, “Phenolic Composition of Kiwi Fruit Juice,” Journal of Agricultural and Food Chemistry, Vol. 47, No. 6, 1999, pp. 2398-2403.
[11] A. Romani, N. Mulinacci, P. Pinelli, F. Vincieri and A. Cimato, “Polyphenolic Content in Five Tuscany Cultivars of Olea europaea L.,” Journal of Agricultural and Food Chemistry, Vol. 47, No. 3, 1999, pp. 964-967.
[12] C. Sanbongi, N. Osakabe, M. Natsume, T. Takizawa, S. Gomi and T. Osawa, “Antioxidative Polyphenols Isolated from Theobroma cacao,” Journal of Agricultural and Food Chemistry, Vol. 46, No. 2, 1998, pp. 454-457.
[13] M. Friedman, “Chemistry, Biochemistry, and Dietary Role of Potato Polyphenols. A Review,” Journal of Agricultural and Food Chemistry, Vol. 45, No. 5, 1997, pp. 1523-1540.
[14] A. Abushita, E. Hebshi and P. Biacs, “Determination of Antioxidant Vitamins in Tomatoes,” Food Chemistry, Vol. 60, No. 2, 1997, pp. 207-212.
[15] O. I. Aruoma, J. Spencer, D. Warren, P. Jenner, J. Butler and B. Halliwell, “Characterization of Food Antioxidants, Illustrated Using Commercial Garlic and Ginger Preparations,” Food Chemistry, Vol. 60, No. 2, 1997, pp. 149156. http://dx.doi.org/10.1016/S0308-8146(95)00254-5
[16] J. Lachman, A. Pronek, A. Hejtmánkova, J. Dudjak, V. Pivec and K. Faitová, “Total Polyphenol and Main Flavonoid Antioxidants in Different Onion (Allium cepa L.) Varieties,” Czech University of Agriculture, Faculty of Agriculture, Prague, 2003.
[17] C. Ganthavorn and J. S. Hughes, “Inhibition of Soybean Oil Oxidation by Extracts of Dry Beans (Phaseolus vulgaris),” Journal of the American Oil Chemists’ Society, Vol. 74, No. 8, 1997, pp. 1025-1030.
[18] F. Markus, H. Daood, J. Kapitany and P. A. Biacs, “Change in the Carotenoid and Antioxidant Content of Spice Red Pepper (Paprika) as a Function of Ripening and Some Technological Factors,” Journal of Agricultural and Food Chemistry, Vol. 47, No. 1, 1999, pp. 100107.
[19] H. Kikuzaki and N. Nakatani, “Antioxidant Effects of Ginger Constituents,” Journal of Food Science, Vol. 58, No. 6, 1993, pp. 1407-1410.
[20] C. Hall and S. Cuppett, “The Hydrogen Donating Mechanism of Rosmariquinone,” American Oil Chemist’s Society 88th Annual Meeting and Exposition, Seattle, 11-14 May 1997, Section 37 General Lipid Oxidation and Quality I.
[21] Ch. Kaur, S. Joshi and H. C. Kapoor, “Antioxidants in Onion (Allium cepa L) Cultivars Grown in India,” Journal of Food Biochemistry, Vol. 33, No. 2, 2009, pp. 184200. http://dx.doi.org/10.1111/j.1745-4514.2009.00212.x
[22] C.-L. Ye, D.-H. Dai and W.-L. Hu, “Antimicrobial and Antioxidant Activities of the Essential Oil from Onion (Allium cepa L.),” Food Control, Vol. 30, No. 1, 2013, pp 48-53.
[23] T. Albishi, J. A. John, A. S. Al-Khalifa and F. Shahidi, “Antioxidant, Anti-Inflammatory and DNA Scission Inhibitory Activities of Phenolic Compounds in Selected Onion and Potato Varieties,” Journal of Functional Foods, Vol. 5, No. 2, 2013, pp. 930-939.
[24] I. Budic-Leto, T. Lovri, I. Pezo and J. Gajdo Kljusuri, “Study of Dynamics of Polyphenol Extraction during Traditional and Advanced Maceration Processes of the Babic Grape Variety,” Food Technology and Biotechnology, Vol. 43, No. 1, 2005, pp. 47-53.
[25] H. Nawaz, J. Shi, G. S. Mittal and Y. Kakudac, “Extraction of Polyphenols from Grape Seedsand Concentration by Ultrafiltration,” Separation and Purification Technology, Vol. 48, No. 2, 2006, pp. 176-181.
[26] Zill-e-Huma, M. Albert-Vian, A.-S. Fabiano-Tixier, M. Elmaataoui, O. Dangles and F. Chemat, “A Remarkable Influence of Microwave Extraction: Enhancement of Antioxidant Activity of Extracted Onion Varieties,” Food Chemistry, Vol. 127, No. 4, 2011, pp. 1472-1480.
[27] G. Cao, E. Sofic and R. L. Prior, “Antioxidant Capacity of Tea and Common Vegetables,” Journal of Agricultural and Food Chemistry, Vol. 44, No. 11, 1996, pp. 34263431.
[28] AOCS (American Oil Chemists’ Society), “Official Method Cd 8-53. Peroxide Value,” In: D. Firestone, Ed., Official Methods and Recommended Practices of the American Oil Chemists’ Society, 5th Edition, AOCS, Champaign, 1998.
[29] “Current Protocols in Food Analytical Chemistry,” John Wiley & Sons, Inc., 2001, D2.1.1-D2.1.15.

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