Leptin Receptor in Ram Epididymal Spermatozoa

Mohammad Roostaei-Ali Mehr, Reza Rajabi-Toustani, Rasool Motamedi-Mojdehi

Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.

DOI: 10.4236/eng.2012.410B031                

Abstract

This experiment was designed to investigate leptin receptors (Ob-R and Ob-Rb) mRNA in ram epididymal spermatozoa by RT-PCR. Ten testes were obtained from abattoir and epididymal spermatozoa recovery was performed. To purify spermatozoa, motile sperm were isolated by the swim-up procedure. Total RNA was isolated from epididymal spermatozoa and placental cotyledon, as a positive control, and then they were purified. Specific bands (98 and 308 bp) for Ob-R and Ob-Rb were detected after RT-PCR in both epididymal spermatozoa and placental cotyledon. We may conclude that Ob-R and Ob-Rb mRNA are present in ram epididymal spermatozoa and leptin perhaps exerts physiological effects, as already demonstrated in human.

Keywords

Epididymal Spermatozoa; mRNA; Ob-R; Ob-Rb

Conflicts of Interest

The authors declare no conflicts of interest.

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