Electronic Detection of Escherichia coli O157︰H7 Using Single-Walled Carbon Nanotubes Field-Effect Transistor Biosensor


Field effect transistors (FET) based on Single-Walled Carbon Nanotubes (SWNTs) become the hot topic in fields of nano-electronic, clinical diagnostics, environmental testing etc. in recent years. In this paper, we reported a simple, scalable way to enrich semiconducting SWNTs by using HNO3/H2SO4. Then carbon nanotube field-effect transistors (CNTFET) biosensor was fabricated with the enrichment SWNTs for Escherichia coli O157H7 detection. The response of each CNTFET was monitored in real time before and after introduction of the Escherichia coli O157H7 at various concentrations. The results show that CNT-FET biosensors we fabricated are sensitive to change of concentration of solution and response time is really short.

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X. Zhang, D. Wang, D. Yang, S. Li and Z. Shen, "Electronic Detection of Escherichia coli O157︰H7 Using Single-Walled Carbon Nanotubes Field-Effect Transistor Biosensor," Engineering, Vol. 4 No. 10B, 2012, pp. 94-98. doi: 10.4236/eng.2012.410B024.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Gruner G, “Carbon nanotube transistors for biosensing applications,” Anal. Bioanal. Chem., vol.384, pp. 322-335, 2006.
[2] Allen B L, Kichambare P D, Star A, “Carbon nanotube field-effect-transistor-based biosensors,”. Adv Mater., vol. 19, pp. 1439-1451, 2007.
[3] Oh J, Yoo S, Chang Y W et al. “Carbon nanotube-based biosensor for detection hepatitis B,” Current Applied Physics, vol. 9, pp. E229-E231, 2009.
[4] Raquel A. Villamizar1, Alicia Maroto and F. Xavier Rius, “Improved detection of Candida albicans with carbon nanotubefield-effect transistors,” Sensors and Actuators B. vol. 136, pp. 451–457, November 2009.?
[5] Villamizar R A., Maroto A, Riusa F.X, Inza I and Figueras M J., “Fast detection of Salmonella Infantis with carbon nanotube field effect transistors,” Biosens. Bioelectron.. vol. 24, pp. 279-283, April 2008.
[6] Martinez M T, Tseng Y C, Ormategui N et al. “Label-free DNA biosensors based on functionalized carbon nanotube field effect transistors,” Nano Letters, vol. 9, pp.530-536,2009.
[7] Besteman K, Lee J O, Wiertz F G M, Heering H A and Dekker C, “Enzyme-coated carbon nanotubes as single-molecule biosensors,” Nano Letters, vol. 3, pp. 727-730, 2003.
[8] Alexander Star,Jean-Christophe P. Gabriel, Keith Bradley, and George Gru1ner, “Electronic detection of specific protein binding using nanotube FET devices,”Nano Letts. vol. 3, pp. 459-463, February 2003.
[9] Li C, Curreli M, Lin H, Lei B, Ishikawa F.N., Datar R, et al, “Complementary detection of prostate-specific antigen using ln2O3 nanowires and carbon nanotubes,” J. Am. Oil Chem. Soc., vol. 127, pp. 12484-12485, 2005.
[10] Bradley K, Briman M, Star A and Gruner G, “Charge transfer from adsorbed proteins,” Nano Letters, vol. 4, pp. 253-256,2004.
[11] Chen C, Zhang W, Zhao B and Zhang Y, “Investigation of Schottky-Barrier carbon nanotube field-effect transistor by an efficient semi-classical numerical modeling,” Phys. Lett. A, vol. 374, pp. 309-312, 2009.
[12] Ralph Krupke, Frank Hennrich, Hilbert v. Lohneysen and Manfred M.Kappes, “Separation of metallic from semi-conducting single walled carbon nanotubes,” Science, vol. 301, pp. 344-347, July 2003
[13] Douglas R. Kauffman, Oleksandr Kuzmych and Alexander Star, “Interactions between single-walled carbon nanotubes and tetraphenyl metalloporphyrins: correlation between spectroscopic and FET measurements,” J. Phys. Chem. C, vol. 111, pp. 3539–3543, 2007.
[14] Zheng M, Jagota A, Semke E D., Bruce A. Diner, Robert S. Mclean, Steve R. Lustig, et al., “DNA-assisted dispersion and separation of carbon nanotubes,” Nat. Mater, vol. 2, pp. 338–342, April 2003
[15] Mark C. HersaM, “Progress towards monodisperse single-walled carbon nanotubes,” Nature Nanotechnology, vol. 3, pp.387-394, July 2008.
[16] Stokes P, Silbar E, Zayas Y M., and Khondaker S I.,“Solution processed large area ?eld effect transistors from dielectrophoreticly aligned arrays of carbon nanotubes,” Appl. Phys. Lett., vol 94, pp. 113104-1-3, March 2009.
[17] Zhang X, Wang D, Yang D, Li S and He Y, “Field-effect Transistor Biosensor Fabricated With Selective Enrichment of Semiconducting Single-Walled Carbon Nanotubes,” ICoBE, pp. 27-28, February 2012.
[18] Yang D, Wang L, Zhao Q and Li S, “Fabrication Of Single-Walled Carbon Nanotubes (SWNTs) Field-Effect Transistor (FET) Biosensor,” BMEI 3rd,vol. 4, pp.1482-1485, 2010.
[19] H.Kataura, Y. Kumazawa, Y. Maniwa, I. Umezu, S. Suzuki, Y. Ohtsuka, et al,, “Optical properities of single-walled carbon nanotubes,” Synth.Met. vol. 103, pp. 2555-2558, 1999.
[20] Francis A. C and Richard J. S, Advantaged Organic Chemistry; McGraw-Hill: New York, 1996.

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