Effect of onion (Allium cepa L.) as an antibiotic growth promoter substitution on performance, immune responses and serum biochemical parameters in broiler chicks


This experiment was conducted to evaluate the effect of onion (Allium cepa L.) as an antibiotic growth promoter substitute on growth performance, immune responses and serum biochemistry in broilers. A total of 192 one-d-old as hatched broiler chicks (Ross 308) were weighed and randomly allocated to four treatment groups, each with 4 replicate pens of 12 chicks. The dietary treatments consisted of the basal diet (control), antibiotic (15 mg Virginiamycin/kg), and control +10 or 30 g fresh onion bulb/kg diet. Body weights of broilers were determined at d 1, 21 and 42, feed intake was determined at the same periods, and feed conversion ratio was calculated accordingly. At 14th and 21st days blood samples were taken for measuring antibody titers against NDV and at 42nd day for biochemical analysis. At d 42, two birds per replicate were slaughtered for determination of lymphoid organ weights. Dietary supplementation of30 g/kg onion increased final body weight of broilers at 42nd d of age compared to the other treatments (P < 0.05). Birds fed30 gonion/kg in the diet had the highest feed intake than other treatments at different growth periods (P < 0.05). Dietary treatments failed to induce any significant effect on antibody titers against NDV, although the weight of lymphoid organs was significantly (P < 0.05) higher for birds fed diets supplemented with30 g/kg Onion. Broilers receiving30 g/kg onion had a significantly higher HDL and lower triglyceride concentrations compared to control groups (P < 0.05). Feeding30 g/kg onion resulted in a marked reduction in the concentration of the glucose compared to control groups (P < 0.05). The results suggested that dietary inclusion of30 g/kg onion can be applied as alternatives to in-feed antibiotics for broiler diets.

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Goodarzi, M. , Landy, N. and Nanekarani, S. (2013) Effect of onion (Allium cepa L.) as an antibiotic growth promoter substitution on performance, immune responses and serum biochemical parameters in broiler chicks. Health, 5, 1210-1215. doi: 10.4236/health.2013.58164.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Landy, N., Ghalamkari, G.H., Toghyani, M., et al. (2011) The effects of Echinacea purpurea L. (Purple Coneflower) as an antibiotic growth promoter substitution on performance, carcass characteristics and humoral immune response in broiler chickens. Journal of Medicinal Plants Research, 5, 2332-2338.
[2] Landy, N. Ghalamkari, G.H. and Toghyani, M. (2011) Performance, carcass characteristics and immunity in broiler chicks fed dietary Neem (Melia azadirachta) as alternative for an antibiotic growth promoter. Livestock Science, 142, 305-309. doi:10.1016/j.livsci.2011.08.017
[3] Burgat, V. (1999) Residues of drugs of veterinary use in food. La Revue du Praticien, 41, 985-990.
[4] Toghyani, M., Toghyani, M., Gheisari, A.A., et al. (2011) Evaluation of cinnamon and garlic as antibiotic growth promoter substitutions on performance, immune responses, serum biochemical and haematological parameters in broiler chicks. Livestock Science, 138, 167-173. doi:10.1016/j.livsci.2010.12.018
[5] Landy, N., Ghalamkari, G.H. and Toghyani, M. (2012) Evaluation of St John’s Wort (Hypericum perforatum L.) as an antibiotic growth promoter substitution on performance, carcass characteristics, some of the immune responses, and serum biochemical parameters of broiler chicks. Journal of Medicinal Plants Research, 6, 510-515. doi:10.5897/JMPR11.1371
[6] Ebesunun, M.O., Popoola, O.O., Agbedana, E.O., et al. (2007) The effect of garlic on plasma lipids and lipoproteins in rats fed on high cholesterol enriched diet. Biokemistri, 19, 53-58.
[7] Melvin, J.M., Jayochitra, J. and Vijayapriaya, M. (2009) Antimicrobial activity of some common spices against certain human pathogens. Journal of Medicinal Plants Research, 3, 1134-1136.
[8] Lampe, J.W. (1999) Health effects of vegetables and fruits: Assessing mechanisms of action in human experimental studies. The American Journal of Clinical Nutrition, 70, 475-490.
[9] Aji, S.B., Ignatius, K., Y. Ado, A. Abdulkarim, et al. (2011) Feeding Onion (Allium cepa) and Garlic (Allium sativum) on some performance characteristics of broiler chickens. Research Journal of Poultry Sciences, 4, 22-27. doi:10.3923/rjpscience.2011.22.27
[10] Sebastian, K., Zacharias, N., Philip, B., et al. (1979) The hypolipidemic effect of onion (Allium cepa Linn) in sucrose fed rabbits. Indian Journal of Physiology and Pharmacology, 23, 27-30.
[11] National Research Council (1994) Nutrient requirements of poultry. 9th Edition, NAS-NRC, Washington DC.
[12] Thayer, S.G. and Beard, C.W. (1998) Serologic procedures. In: Swayne, D.E., Ed., A Laboratory Manual for the Isolation and Identification of Avian Pathogens, 4th Edition, American Association of Avian Pathologists, Philadelphia, 256-258.
[13] Shurlock, T.G.H. and Forbes, J.M. (1981) Evidence for hepatic glucostatic regulation of food intake in the domestic chicken and its interaction with gastro-intestinal control. British Poultry Science, 22, 333-346. doi:10.1080/00071688108447893
[14] AL-Homidan, A.A. (2005) Efficacy of using different sources and levels of Allium cepa, Allium sativum and Zingiber officinale on broiler chicks performance. Saudi Journal of Biological Sciences, 12, 96-102.
[15] Ibrahiem A.I., Talib, A.E., Fathi, F.M., et al. (2004) Effect of onion and/or garlic as feed additives on growth performance and immunity in broiler muscovy ducks. First Scientific Conference of Faculty of Veterinary Medicine, Benhar, 1-4 September 2004.
[16] Yamamoto, Y. and Glick, B. (1998) A comparison of the immune response between two lines of chickens selected for differences in the weight of the bursa of Fabricius. Poultry Science, 61, 2129-2132. doi:10.3382/ps.0612129
[17] Dafwang, L.M.E., Sunde, M.L. and Bird, H.R. (1985) Bursal, intestinal and spleen weight and antibody response of chicks feed substance therapeutic levels of dietary. Poultry Science, 64, 634-639. doi:10.3382/ps.0640634
[18] Coates, M.E., Davies, M.K. and Kon, S.K. (1952) The effect of antibiotics on the intestine of the chick. British Journal of Nutrition, 9, 110-119. doi:10.1079/BJN19550016
[19] Whitehair, C.K. and Thompson, C.M. (1956) Observations in raising “disease-free” swine. Journal of the American Veterinary Medical Association, 128, 94-98.
[20] Suresh Babu, P. and Srinivasan, K. (1997) Influence of dietary capaicin and onion on the metabolic abnormalities associated with streptozotocin induced diabetes mellitus. Molecular and Cellular Biochemistry, 175, 49-57. doi:10.1023/A:1006881027166
[21] Sklan, D., Bernera, Y.N. and Rabinowitch, H.D. (1992) The effect of dietary onion and garlic on hepatic lipid concentrations and activity of antioxidative enzymes in chicks. The Journal of Nutritional Biochemistry, 3, 322-325. doi:10.1016/0955-2863(92)90022-B

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