Flash microbiocide: A Rapid and Economic Method for Determination of MBC and MFC

Camila Hernandes, Juliana da Silva Coppede, Bianca Waléria Bertoni, Suzelei de Castro França, Ana Maria Soares Pereira

Unity of Biotechnology, University of Ribeirao Preto, Ribeir?o Preto, Brazil.

DOI: 10.4236/ajps.2013.44104                

Abstract

Although nowadays there are methods for determining the Minimal Bactericidal Concentration and Minimal Fungicidal Concentration, it is indispensable that the establishment of innovative methodologies could be more practical and cheaper. The new methodology Flash microbiocide is an assay in which one aliquot from 96 well plate of Minimal Inhibitory Concentration test is transferred to another plate containing different culture medium. The correspondence with the reference methods described in the National Committee for Clinical Laboratory Standards (NCCLS-CLSI) document M26-A was achieved, denoting the efficiency of this fast and simple method.

Keywords

Antimicrobial Activity; Minimal Inhibitory Concentration; Minimal Bactericidal Concentration; Minimal Fungicidal Concentration; Methodology

Conflicts of Interest

The authors declare no conflicts of interest.

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