Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.)


Both morphological characters and PCR-based RAPD approaches were used to determine the genetic diversity and relatedness among nine fig genotypes grown at the northern region of the West-Bank, Palestine. Although we tested 28 primers for the RAPD technique, only 9 produced reasonable amplification products. A total of 57 DNA loci were detected in which 70.2% were polymorphic. DNA fragments presented a minimum of 3 and a maximum of 9 polymorphic bands using primers OPT-10 and OPA-18, respectively. Primers exhibited collective resolving power values (Rp) of 18.826. The Mwazi genotype showed the highest genetic distances among all of the other genotypes. Morphologically, considerable variations were found using 41 quantitative and qualitative traits. Adloni could be a very promising genotype for fresh consumption due to its very late maturation period, extended harvesting period, variable fruit size, and easy skin peeling. In addition, 7 genotypes presented firm fruits, which are a very important criterion for exporting purposes. Dendrogram constructed by UPGMA based on RAPD banding patterns appear somewhat contradictory to the morphological descriptors particularly with Swadi and Biadi genotypes (closed genetically and distanced morphologically), which might be attributed to the phenotypic modifications caused by environmental differences across regions. These preliminary results will make a fundamental contribution to further genetic improvement of fig crops for the region.

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R. Basheer-Salimia, M. Awad and J. Ward, "Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.)," American Journal of Plant Sciences, Vol. 3 No. 9, 2012, pp. 1241-1251. doi: 10.4236/ajps.2012.39150.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] M. Flaishman, V. Rodov and E. Stover, “The Fig: Botany, Horticulture, and Breeding,” Horticultural Reviews, Vol. 34, 2008, pp. 113-197.
[2] FAO, “The FAO Statistical Database-Agriculture,” 2009. book-2009/en/.
[3] F. Aljane and A. Ferchichi, “Assessment of Genetic Diversity among Some Southern Tunisian Fig (Ficus carica L.) Cultivars Based on Morphological Descriptors,” Jordan Journal of Agricultural Sciences, Vol. 5, No. 1, 2009, pp. 1-16.
[4] M. E. Kislev, A. Hartmann and O. Bar-Yosef, “Early Domesticated Fig in the Jordan Valley,” Science, Vol. 312, No. 5778, 2006, pp. 1372-1374. doi:10.1126/science.1125910
[5] M. Aradhya, E. Velasco and A. Koehmstedt, “Genetic Structure and Differentiation in Cultivated Fig (Ficus carica L.),” Genetica, Vol. 138, No. 6, 2010, pp. 681-694. doi:10.1007/s10709-010-9442-3
[6] O. Caliskan and A. A. Polat, “Morphological Diversity among Fig (Ficus carica L.) Accessions Sampled from the Eastern Mediterranean Region of Turkey,” Turkish Journal of Agriculture and Forestry, Vol. 36, 2012, pp. 179-193.
[7] M. T. Sadder and A. F. Atteyyeh, “Molecular Assessment of Polymorphism among Local Jordanian Genotypes of The Common Fig (Ficus carica L.),” Scientica Horticulturae, Vol. 107, No. 4, 2006, pp. 347-351. doi:10.1016/j.scienta.2005.11.006
[8] G. R. Rout and A. Mohapatra, “Use of Molecular Markers in Ornamental Plants: A Critical Reappraisal,” European Journal of Horticultural Science, Vol. 71, No. 2, 2006, pp. 53-68.
[9] O. Saddoud, G. Baraket, K. Chatti, M. Trifi, M. Marrakchi, A. Salhi-Hannachi and M. Mars, “Morphological Variability of Fig (Ficus carica L.) Cultivars,” International Journal of Fruit Science, Vol. 8, No. 1-2, 2008, pp. 35-51. doi:10.1080/15538360802365921
[10] A. Salhi-Hannachi, K. Chatti, O. Saddoud, M. Mars, A. Rhouma, M. Marrakchi and M. Trifi, “Genetic Diversity of Different Tunisian Fig (Ficus carica L.) Collection Revealed by RAPD Fingerprints,” Hereditas, Vol. 143, 2006, pp. 15-22. doi:10.1111/j.2005.0018-0661.01904.x
[11] M. Podgornik, I. Vuk, I. Vrhovnik and D. B. Mavsar, “A Survey and Morphological Evaluation of Fig (Ficus carica L.) Genetic Resources from Slovenia,” Scientia Horticulturae, Vol. 125, No. 3, 2010, pp. 380-389. doi:10.1016/j.scienta.2010.04.030
[12] M. L. A. Lima, A. A. F. Garcia, K. M. Matsuoka, H. Arizono and C. L. De-Souza, “Analysis of Genetic Similarity Detected by AFLP and Coefficient of Percentage among Genotypes of Sugar Cane (Saccharum spp.),” Theoretical and Applied Genetics, Vol. 104, No. 1, 2002, pp. 30-38. doi:10.1007/s001220200003
[13] M. Agarwal, N. Shrivastava and H. Padth, “Advances in Molecular Markers Techniques and Their Applications in Plant Sciences,” Plant Cell Reports, Vol. 27, No. 4, 2008, pp. 617-631. doi:10.1007/s00299-008-0507-z
[14] B. Khadari, P. H. Lashermes and F. Kjellberg, “RAPD Fingerprints for Identification and Genetic Characterization of Fig (Ficus carica L.) Genotypes,” Journal of Genetics and Breeding, Vol. 49, 1995, pp. 77-86.
[15] B. Khadari, I. Hochu, S. Santoni, A. Oukabli, M. Ater, J. P. Roger and F. Kjellberg, “Which Molecular Markers are Best Suited to Identify Fig Cultivars: A comparison of RAPD, ISSR and Microsatellite Markers,” Acta Horticulturae, Vol. 605, 2003, pp. 69-75.
[16] P. J. Elisiario, M. C. Neto, L. F. Cabrita and J. M. Leitao, “Isoenzyme and RAPDs Characterization of a Collecion on Fig (Ficus carica L.) Traditional Varieties,” Acta Horticulturae, Vol. 480, 1998, pp. 149-154.
[17] U. Galderisi, M. Cipollaro, G. Di Bernardo, L. De Masi, G. Galano and A. Cascino, “Identification of the Edible Fig ’Bianco del Cilento’ by Random Amplified Polymorphic DNA Analysis,” HortScience, Vol. 3, 1999, pp. 1263-1265.
[18] L. F. Cabrita, U. Aksoy, S. Hepaksoy and J. M. Leitao, “Suitability of Isozyme, RAPD and AFLP Markers to Assess Genetic Differences and Relatedness AmongFig (Ficus carica L.) Clones,” Scientia Hortculturae, Vol. 87, No. 4, 2001, pp. 261-273. doi:10.1016/S0304-4238(00)00181-3
[19] K. Papadopoulou, C. Ehaliotis, M. Tourna, P. Kastanis, I. Karydis and G. Zervakis, “Genetic Relatedness among Dioecious Fig (Ficus carica L.) Cultivars by Random Amplified Polymorphic DNA Analysis, and Evaluation of Agronomic and Morphological Characters,” Genetica, Vol. 114, No. 2, 2002, pp. 183-194. doi:10.1023/A:1015126319534
[20] Y. Aka-Kacar, A. B. Kuden and S. Cetiner, “Identification of Varietal Polymorphism in Ficus carica L. by RAPD (Randomly Amplified Polymorphic DNA) Markers,” Acta Horticulturae, Vol. 598, 2003, pp. 167-172.
[21] L. De Masi, M. Cipollaro, G. Di Bernardo, U. Galderisi, G. Galano, A. Cascino, G. Grassi, E. Pavone and A. Simeone, “Clonal Selection and Molecular Characterization by RAPD Analysis of The Fig (Ficus carica L.) ‘Dottato’ and ‘Blanco del Cilento’ Cultivars in Italy,” Acta Horticulturae, Vol. 605, 2003, pp. 65-68.
[22] H. Achtak, A. Oukabli, M. Ater, S. Santoni, F. Kjellberg and B. Khadari, “Microsatellite Markers as Reliable Tools for Fig Cultivar Identi?cation,” Journal of American Society for Horticultural Science, Vol. 134, No. 6, 2009, pp. 624-631.
[23] M. Kocsis, L. Jaromi, P. Putnoky, P. Kozma and A. Borhidi, “Genetic Diversity among Twelve Grape Cultivars Indigenous to the Carpathian Basin Revealed by RAPD Markers,” Vitis, Vol. 44, No. 2, 2005, pp. 87-91.
[24] M. Hoogendijk and D. E. Williams, “Characterizing the Genetic Diversity of Home Garden Crops: Some Examples from the Americas,” In: J. W. Watson and P. D. Eyzaguirre, Eds., Home Gardens and in situ Conservations of Plant Genetic Resources in Farming Systems, Proceeding of the Second International Home Gardens Workshop, Witzenhausen, 17-19 July 2001, pp. 34-40.
[25] C. Cantini, A. Cimato and G. Sani, “Morphological Evaluation of Olive Germplasm Present in Tuscany Region,” Euphytica, Vol. 109, No. 3, 1999, pp. 173-181. doi:10.1023/A:1003728800464
[26] A. Prevost and M. J. Wilkinson, “A New System of Comparing PCR Primers Applied to ISSR Fingerprinting of Potato Cultivars,” Theoretical and Applied Genetics, Vol. 98, No. 1, 1999, pp. 107-112. doi:10.1007/s001220051046
[27] J. E. Gilbert, R. V. Lewis and M. J. Wilkinson, “Developing an Appropriate Strategy to Assess Genetic Variability in Plant Germplasm Collections,” Theoretical and Applied Genetics, Vol. 98, No. 6-7, 1999, pp. 1125-1131. doi:10.1007/s001220051176
[28] P. M. Schluter and S. A. Harris, “Analysis of Multilocus Fingerprinting Data Sets Containing Missing Data,” Molecular Ecology Notes, Vol. 6, No. 2, 2006, pp. 569-572. doi:10.1111/j.1471-8286.2006.01225.x
[29] IPGRI and CIHEAM, “Descriptors for Fig (Ficus carica L.),” International Plant Genetic Resources Institute (IPGRRI), Rome, Italy and International Center for Advanced Mediterranean Agronomic Studies (CIHEAM), Paris, 2003.
[30] M. Akbulut, S. Ercisli and H. Karlidag “RAPD-Based Study of Genetic Variation and Relationships among Wild Fig Genotypes in Turkey,” Genetics and Molecular Research, Vol. 8, No. 3, 2009, pp. 1109-1115. doi:10.4238/vol8-3gmr634
[31] D. Bandelj, J. Jakse and B. Javornik, “DNA Fingerprinting of Olive Varieties by Microsatellite Markers,” Food Technology and Biotechnology, Vol. 40, 2002, pp. 185-190.
[32] G. Baraket, K. Chatti, O. Saddoud, A. Ben-Abdelkarim, M. Mars, M. Trifi and A. Hannachi, “Comparative Assessment of SSR and AFLP Markers for Evaluation of Genetic Diversity and Conservation of Fig, Ficus carica L., Genetic Resources in Tunisia,” Plant Molecular Biology Reporter, Vol. 29, No. 1, 2011, pp. 171-184.
[33] L. Chalak, A. Chehade, E. Mattar and B. Khadari, “Morphological Characterization in Fig Accessions Cultivated in Lebanon,” Acta Horticulturae, Vol. 798, 2008, pp. 49-56.
[34] O. Caliskan and A. A. Polat, “Fruit Characteristics of Fig Cultivars and Genotypes Grown in Turkey,” Scientica Horticulturae, Vol. 115, No. 4, 2008, pp. 360-367. doi:10.1016/j.scienta.2007.10.017
[35] M. Simsek, “Fruit Performances of the Selected Fig Types in Turkey,” African Journal of Agricultural Research, Vol. 4, 2009, pp. 1260-1267.
[36] F. Aljane, “Analyse de la Diversité Génétique de Cultivars Locaux du Figuier (Ficus carica L.) Cultivés Dans la Cha?ne de Matmata,” Rev. des Rég. Arides, 2004, pp. 95-104.
[37] A. Oukabli, A. Mamouni, M. Laghezali and B. Khadari, “Genetic Variability in Moroccan Fig Cultivars (Ficus carica L.) Based on Morphological and Pomological Data,” Acta Horticulturae, Vol. 605, 2003, pp. 311-318.

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